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    Bulletin of Botanical Research 1999 Vol.19
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    A NEWSECTION OF THE GENUS OXYTROPIS DC.(PAPILIONACEAE)
    Qian Guan-ze, Liu Lian-fen, Zhu Qi, Song Xing-min
    Bulletin of Botanical Research    1999, 19 (1): 1-4.  
    Abstract350)      PDF(pc) (160KB)(346)       Save
    A section of the Genus Oxytropis DC.-Sect. Ochrocea G. Z. Qian is recorded in this paper. At present, two species are included in this section. They are O. ochrantha Turcz. and O. viridiflava Kom.. In the past, O. chrysotricha Franch. was regarded as O. ochrantha Turcz. in works of Chinese scholars. The author pointed out that it should be a variety of O. ochrantha Turcz., and treated the forma O. ochrantha Turcz. f. diversicolor H. C. Fu et Ma as a variety. At last, the species O. viridif lava Kom. is a new distribution species in China.
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    A NEW SPECIES OF SYMPLOCOS FROM YUNNAN
    Qian Yi-yong
    Bulletin of Botanical Research    1999, 19 (1): 5-7.  
    Abstract233)      PDF(pc) (137KB)(149)       Save
    The paper reports a new species-Symplocos menglianensis Y. Y. Qian
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    TWO NEW VARIETYS OF THE GENUS HEDYSARUM FROM QINGHAI, CHINA
    WuYu-hu
    Bulletin of Botanical Research    1999, 19 (1): 8-9.  
    Abstract249)      PDF(pc) (87KB)(256)       Save
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    PRELIMINARY STUDIES ON THE FLORA OF THE BAOTIANMAN NATURAL RESERVE
    Zhang Nai-qun
    Bulletin of Botanical Research    1999, 19 (1): 10-16.  
    Abstract201)      PDF(pc) (258KB)(380)       Save
    The Baotianman Natural Reserve is located in transitional zone from north subtropic to south warm temperate in the southern slope of Funiushan Mountain. There are 2771 species of higher plants which belong to 223 families and 1002 genera. The flora is complex in geographical elements, having 15 types and 17 subtypes. Through the analysis to the families and genera, the characters of the flora of the Baotianman Natural Reserve are mainly of temperate zone and shows a crsscross and certain old.
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    A STUDY ON THE POLLEN MORPHOLOGY OF THE POLYGONACEAE IN ANHUI PROVINCE
    Zhou Zhong-ze, Lu Run-long, Ge Lei, Bao Shi-lai, Zheng Yan-yan
    Bulletin of Botanical Research    1999, 19 (1): 17-33.  
    Abstract357)      PDF(pc) (6335KB)(275)       Save
    Pollen morphology of 46 species and 3 varieties representing the genera i.e. Polygonum, Antenoron, Fagopyrum, Rheum, Rumex, Homalocladium was examined in the light microscope (LM) and with the scanning electron microscope (SEM). Pollen grains of Polygonaceae in Anhui Province are spheroidal or subspheroidal, prolate or subprolate; 3-colpate, 12-pantocolpate, 30-colporate, 3-colporate, 4-colporate, 9-pantocolporate, 20-30-pantoporate. The ornamentation of exine is finely or coarsely reticulate, rugulate, granulate-perforate, punctate, microechinate-perforate-smooth, granulate-perforate-smooth, granulate-foveolate, prominently sunken punctate, prominently punctate-striate, densely covered granulate. Based on the characteristics, seventeen pollen types in the family Polygonaceae of Anhui province are recognized and described, i.e. Cephalophilon-type, Tovara-type, Amphibium-type, Persicaria-type, Macranthum-type, Bistorta-type, Avicularia-type, Plebeium-type, Convolvulus-type, Tiniaria-type, Cuspidatum-type, Fagopyrum-type, Sibiricum-type, Rheum-type, Acetosa-type, Aquaticus-type, Homalocladium-type, among which, four new pollen types are proposed, i.e. Macranthum-type, Plebeium-type, Cuspidatum-type, Homalocladium. A key to the types is provided. According to the pollen types ,the authors disagree with wang Jian-Xing's treatment which divided the four species, i.e. Polygonum multiflorum, P. cuspidatum, P. convolvulus, P. dentato-alatum into two sections, and consider the division of the four species into three sections.
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    STUDIES ON THE POLLEN MORPHOLOGY OF KALIMERIS
    Wang Jing, Zhang Xiao-ping, Fang Yu-da
    Bulletin of Botanical Research    1999, 19 (1): 34-39.  
    Abstract242)      PDF(pc) (2332KB)(265)       Save
    Pollen grains of nine species, three varieties and one form variation of the genus Kalimeris (Compositae) have been examined under light microscope (LM) and scanning electron microscope (SEM). All the species examined are characterized in having sub-globular pollen grains and tripartite global pollen grains on AMB. The size of the pollen grains ranges from 20.4 μm to 32.3 μm in the polar axis and from 20.4 μm to 34.6 μm in the longest equatorial axis. The ornamentation of the exine are splinter-shaped under SEM. The pollen grains are diverse in the size and the ornamentation of the exine, these differences can be taken as the base of identification. Kalimeris indica var. polymorpha has special ornamentation of the exine and can be elevated to the specifical level. Kalimeris shimadai f. pinnatifida is regarded as conspecies with Kalimeris pinnatifida based on the characters of leaves and pollen grains. Aster procerus and Aster smithianus are transferred to Kalimeris based on the characters of pollen grains and morphology, which gives support Hu Xiuying's taxonomic treatment.
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    THE DEVELOPMENTOF EMBRYO AND ENDOSPERM IN ELEUTHEROCOCCUS BRACHYPUS
    Wang Zhong-li, Liu Lin-de, Tian Guo-wei, Shen Jia-heng
    Bulletin of Botanical Research    1999, 19 (1): 40-47.  
    Abstract261)      PDF(pc) (858KB)(289)       Save
    The development of embryo of Eleutherococcus brachypus belongs to Solanad type.In the late stick embryo stage, the suspensor is most developed.In the globular embryo stage, the suspensor begins to degenerate and its disintegration occurs in the stage of heart embryo.Endosperm is nuclear type.In the stage of about 200~300 free nuclei, endosperm begins to celluarize and its number of cells further increase by mitosis.In the globular embryo stage, the most outer layer of endosperm is specialized to secretory layer.Endosperm cells reserve protein and fat.The most inner layer of single integument is differentiated into endothelium in the stage of 32~64 free nu-clei.After division of zygote, the endothelium fully developed and it is pressed in the late stage of stick embryo.In the globular embryo stage, endo thelium degenerates and the outer layer of integument specialized into seed vessel.
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    CO 2 RESPONSESOF PHYSIOLOGICAL ECOLOGY CHARACTERISTICS IN LARIX OLGENSIS HENRY AND ITS SIGNIFICANCE
    Feng Yu-long, Wang Li-hua, Ao Hong, Nie Jiang-li, Wang Wen-zhang
    Bulletin of Botanical Research    1999, 19 (1): 53-59.  
    Abstract346)      PDF(pc) (276KB)(216)       Save
    Net photosynthetic rate, transpiration rate and water utilization efficiency of 2-year-old cutting seedlings of different clones of Larix olgensis Henry growing in phytotron, were measured at different CO 2 concentration, and regressive analysis was done.The results showed that net photosynthetic rate and water utilization efficiency increased, while transpiration rate decreased, with the increasement of CO 2 concentration.But the parttern and intensity of CO 2 response of different clone were different.CO 2 compensation point of different clone of Larix olgensis Henry was different either.These diversity were the fundation of clone selection.CO 2 respones of net photosynthetic rate and water utilization efficiency had very important significance in clone selection, while CO 2 compensation point was senseless.Growth of larix olgensis Henry might become faster and its distribution area might become bigger in future higher CO 2 concentration condition.
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    A CHARACTER STUDY ON CHLOROPHYLL OF ARCTIUM LAPPA LINN.
    Yuan Xiao-ying, Wang Ping, Yang Chun-yu
    Bulletin of Botanical Research    1999, 19 (1): 60-63.  
    Abstract221)      PDF(pc) (164KB)(216)       Save
    The character of chlorophyll of Arctium lappa Linn. was studied in the paper. The purpose of research is providing some reliable information for further processing. Items such as content of chlorophyll and relationship between chlorphyll and light, temperature and food additives were given. Results showed that the content of thlorophyll was 2.3195 wg/g. The speed of chlorophyll reduction and decomposition was quicker and quicker as time went under dispersing light in laboratory. When heated the speed of chlorophyll reduction and decomposition was also quicker and quicker as temperature was higher and higher. Beside sugar, the additives play more of less role to the pigment. So proper methods should be adopted in production.
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    EFFECTS OF EMS & 60Co ON SEED GERMINATION OF CRAMBE ABYSSINICA AND ITS AGRONOMIC CHARACTER OF M 1 GENERATION
    Wang You-ping, Xu Xiao-xia, Gao Hong-bo, Lan Le-fu
    Bulletin of Botanical Research    1999, 19 (1): 64-67.  
    Abstract201)      PDF(pc) (174KB)(183)       Save
    Physiological process of germinating Crambe seeds was significantly influenced by the treatment of different concentration and dose of EMS 60Co. Low-level of them could stimulate the respiration and raise the activities of ascorbic acid oxidase and peroxidase which associated with higher rate of seed germination and growth of seedling. High-level had inhibiting effects on respiration and the activities of related enzymes, the germinating potential of seed and the rate of germination and the vigor of root system decreased. The yield per plant and the resistance to disease of M 1 generation of Crmbe abyssinica were also influenced by the treatment of different concentration and dose of EMS 60Co.
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    STUDY ON THE EFFECT OF TOMATO SEEDS PHYSIOLOGY AND BIOCHEMISTRY WITH MAGNETIC FIELD TREATMENT
    Mi Xiao-ju, Ma Yue, Guo Gui-yun
    Bulletin of Botanical Research    1999, 19 (1): 68-74.  
    Abstract224)      PDF(pc) (257KB)(260)       Save
    After treatment tomato seeds with magnetic field, observed the germination rate, the change of the catalase (CAT)and peroxidase (POD) and the effect on the peroxidase isoenzyme.The results revealed that the seeds germination rate is highest, the activity of the catalase (CAT) and the peroxidase (POD)is highest. The activity of the peroxidase isoenzyme is highest too, when magnetic field is in range 1000~2000 GS.The seeds germination rate and the activity of CAT and POD is lower, when the intensity of magnetic field is higher than 3000 GS.
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    ALLOZYME ANALYSIS ON NATURAL POPULATION FOR PINUS KORAIENSIS
    Zu Yuan-gang, Zhang Heng-qing, Yan Ting-fen, Yuan Xiao-ying
    Bulletin of Botanical Research    1999, 19 (1): 75-79.  
    Abstract190)      PDF(pc) (210KB)(213)       Save
    Ten enzyme systems were tested for the endosperm of korean pine from three populations using the horizontal starch gel electrophoresis technique. 18 loci were obtained. The results indicated the ratio of polymorphic loci was 77.78%, the average number of alleles was 2.0(A) and the expected heterozygous was 0.1648(He). The genetic differentiation (Gst=5.38%) among populations was lower than other species in Pinus genus and most variations were within population. According to Nei's index, the genetic distance (0.0095) between Xiaobeihu and Changbai Mountain was nearest, not correlation to the geographical distance. It shows the original complexity of population.
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    COMPARATIVE ANATOMY OF THE SECONDARY PHLOEM OF TAXACEAE
    Han Li-juan, Hu Yu-xi, Lin Jin-xing
    Bulletin of Botanical Research    1999, 19 (1): 80-87.  
    Abstract209)      PDF(pc) (1244KB)(313)       Save
    The family Taxaceae contains five genus, Taxus, Pseudotaxus, Amentotaxus, Torreya, Austrotaxus, except for Austrotaxus mastly endemic to China. The prensent paper deals with the comparative anatomy of secondary phloem of stems in 8 species of five genus and light microscope and scanning electron microscope. The main results are as follows: The secondary phloem of Taxaceae is composed of axial and radial systems. In the axial system the phloem consists of sieve cells, phloem parenchyma cells, albuminous cells and phloem fibers. In the radial systemit consists of phloem rays. In cross section the layer of consists elements are different. The forms, size, quantity, have or not of crystal and the forms of crystal of phloem fibers are different. This can be served as the characters for identification of genus and also provding the evideuce for the tribes in the family. Based on the fact that crystals are absenton the outer surface of phloem fibers in Austrotaxus and present on the fibers of other four genera. The forms, size and frequency of crystals on the wall surfaces of the phloem fibers in four genera of Taxaceae are different. It can be divided into four tribes: Taxeae (including Taxus and Pseudotaxus), Austrotaxeae ( Austrotaxus),Amentotaxeae ( Amentotaxs) and Torreyeae ( Torreya).
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    ANALYSIS ON THE DYNAMIC OF ABOVEGROUND BIOMASS AND TIME SEQUENCE OF DIFFERENT DEYEUXIA ANGUSTIFOLIA COMMUNITIES IN SANJIANG PLAIN
    Ni Hong-wei
    Bulletin of Botanical Research    1999, 19 (1): 88-93.  
    Abstract220)      PDF(pc) (228KB)(183)       Save
    D. angustifoia is the dominant species in the typical meadow, marsh meadow and marsh communities of Sanjiang plain. According to these, this paper researched the dynamic of aboveground biomass and time sequence in D. angustifolia. The result reveals that: The seasonal dynamic of aboveground biomass in the three communities was monocurve and the maximum was in the end of July. The changing law of seasonal dynamic of aboveground biomass has been revealed by time sequence.
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    THE INFLUENCEOF GAPS TO THE HERB
    Zhang Yan-hua, Nie Shao-quan, Wang Zhi-xi
    Bulletin of Botanical Research    1999, 19 (1): 94-99.  
    Abstract291)      PDF(pc) (226KB)(270)       Save
    In the course of restoration of the secondary broad-leaved forest, the gaps which existing in the forest with different areas had been reformed by the gap technique and had yielded good results.It not only made the aim species growing up well in the gaps and promoted the growth of broad-leaved trees which surrounding the gaps, but also changed herbs in many respects such as species, density and cover degree.
    In this paper, we reseach on herbs which in gaps and under the broad-leaved trees.The results showed that light is a key factor to the herb's growth and distribution.It is of great immediate significance that the gaps will promote the herb's growth and enhance the species diversity.
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    STATUS AND ROLE OF THE SHRUB LAYER AND ITS MAIN SHRUB SPECIES IN NUTRIENT CYCLING OF MONGOLIAN OAK FOREST
    Guan Ji-yi, Chen Yi-liang, Zhu Ning, Zhuo Li-huan, Liu Yi
    Bulletin of Botanical Research    1999, 19 (1): 100-110.  
    Abstract274)      PDF(pc) (407KB)(332)       Save
    The status and role of the shrub layer and main shrub species in the nutrient cycling of the mongolian oak forest were stated in this paper. The results show that the shrub layer's cycling rate is 0.68 which was more than the arbor's (0.6) and the forest's (0.64) although it is less important than the arbor layer in biomass, nutrient content, remained content, returned content etc. The shrub layer have a important role in promoting the nutrient cycling of the forest. Corylns mandshurica and Deutyia amurensis are dominant or Sub-dominat shrub species which should be protected because they Play an important role in the nutrient cycling, Acanthopanax senticasus matains more nutrient and the cycling rate is low in the nutrient cycling. It exhausted the soil but can be used as a kind of drug. So it should be protected and on the other hand should be controled.
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    COMPARATIVE STUDY ON MORPHOLOGY OF ARTEMISIA PLANTS FROM NORTHEASTERN CHINA I. MORPHOLOGY OF SEED COAT
    Zhang Da-wei
    Bulletin of Botanical Research    1999, 19 (1): 111-120.  
    Abstract268)      PDF(pc) (3538KB)(157)       Save
    The morphology of seed coat from 35 representative species in groups of Sect. Absiuthium DC., Sect. Abrotanum Bess., Sect. Artemisia, Sect. Dracunculus Bess and Sect. Iatilobus Y. R. Ling from Artemisia were observed using both optical microscope and scanning electron microscope. The shape of most achenes was inversely-ovate, lengthened-round or in other variable forms. However, the stereoscopic configuration varied to certain extent. All the epidermal cells of achenes had secondary thickening. First of all, the vertical and the horizontal perines of epidermal cells thickened extremely and they protruded so as to interconnect as netted structure. Secondly, the relatively intensive secondary thickening took place in the cell wall of the seed coat so that the concentrated longitudinal ridges formed on the surface of seed. Certain variations oceurred interms of the diameter and the layer number of the collar cells. The difference in these microstructure characters among groups may be of importance in plant taxonomy and ecology.
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    FUNARIA AMERICANA LINDB. EX SULL. NEW TO CHINA AND EURASIAN CONTINENT
    Zhao Jian-cheng, Li Min, Maimtiming Sulayman
    Bulletin of Botanical Research    1999, 19 (2): 1-3.  
    Abstract313)      PDF(pc) (158KB)(191)       Save
    Funaria americana Lindb. ex Sull. is reported new to the Xiao Wutai Mountain (114°47'~115°30'E,39°50'~40°07'N),Taihang Mountains in the Hebei province of China, a locality widely disjunctive from its previously known distribution in the North America. This is its first report from the Eurasian continent. The specimens are deposited in the herbarium of Hebei Normal University (HBNU).
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    SOME NEW RECORDED PLANTS ON HAINAN ISLAND
    Shan Jia-lin
    Bulletin of Botanical Research    1999, 19 (2): 4-6.  
    Abstract279)      PDF(pc) (120KB)(395)       Save
    2 genera, 6 species and 3 varieties are reported as new records of flora on Hainan island in this paper, and one new variety is also reported here. All the voucher specimens are preserved in herbarium of South China University of Tropical Agriculture.
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    NUMERICAL TAXONOMIC STUDIES ON SOLANUM NIGRUM IN CHINA
    Zhang Hai-yang, Jiang Xiang-jun, Dong Xi-wen, Han Qiu-ju
    Bulletin of Botanical Research    1999, 19 (2): 7-11.  
    Abstract213)      PDF(pc) (197KB)(245)       Save
    Based on studing external morphological characters of Solanum nigrum L., S. nigrum L. var. flavovirens S. Z. Liou et W. Q. Wang and S. nigrum L. var. pauciflorum Liou in china have been studied. The difference in colour of ripe fruit and leaf shape of S. nigrum L. var. flavovirens S. Z. Liou et W. Q. Wang and S. nigrum L. has been found, which is in colour of ripe fruit and trichome shape and size of organ of S. nigrum L. var. pauciflorum Liou and S. nigrum L.. Through cluster analysis, S. nigrum L. var. pauciflorum Liou is one class. S. nigrum L. and S. nigrum L. var. flavovirens S. Z. Liou et W. Q. Wang are another class. S. nigrum L. var. pauciflorum Liou shows primitive character, which has been considered to be an independent species, S. pauciflorum (Liou) H. Y. Zhang. S. nigrum L. and S. nigrum L. var. flavovirens S. Z. Liou et W. Q. Wang are different polyploid species which evoluted from S.nigrum L. var. pauciflorum Liou.
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    THE LEAF EPIDERMIS OF HELMINTHOSTACHYACEAE AND OPHIOGLOSSACEAE
    Jiao Yu, Chen Li-qun, Song Shu-yin
    Bulletin of Botanical Research    1999, 19 (2): 12-15.  
    Abstract291)      PDF(pc) (290KB)(322)       Save
    The outer and inner surfaces of leaf epidermis of three genera ( Heminthostachys, Ophioderma, Ophioglossum) in Heminthostachyaceae and Ophioglossaceae were investigated under light and scanning electron microscopes. Their epidermal cells are regular with straight or slightly curved anticlinal walls. The longitudinal axes of stomatal apparatus generally parallel with the long axis of the lamina. Guard cells somewhat sunken. The epidermal features of the three genera are different in the outline of epidermal cells, the degree of curved anticlinal wall of epidermal cells, the distribution and types of stomatal apparatus. These differences contribute evidences for systematics of Ophioglossales.
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    THE THEORY OF COTYLODON NODE ZONE AND THE EVOLUTION OF THE STRUCTURE OF WOOD
    Gu Yi, Zhao Li-hui, Gu An-gen
    Bulletin of Botanical Research    1999, 19 (2): 16-22.  
    Abstract302)      PDF(pc) (281KB)(219)       Save
    From the phyllogeny, We found that the Wood Which came first should be gryptogamic wood of the arboreous ferns, such as Lopidodondron Sphenophyllum, etc, which were extinet long before. Those which survived are all phanegamic wood, including conifer wood of Gymnosperm and broad-leaved wood of Angiosperm, which appeared after the palaeozoic era and Mesozoic era. The Gryptogamic wood are primary and have exarch and mezarch Primary xylem; while the phanegamic wood are evolutionary and have endarch Primary Xylem (with exleption). After Studying on the cotyledon no des of the Seedlings of Angiosperm, we have found that between the cotyledon node and epicotyl shool zone there are still meristimatic tissues which have no relations with moristem on tap. In the splitting process, these tissues can control the split of the primary vascular tissues and make them evolve from mezarch primary Xylem of seed ferms to endarch primary Xylem of Gymnosperm and Angiosperm. Thus, the secondary phanegamic wood can be evolved from the Primary Gryptogamic wood.
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    MORPHOLOGICAL STUDY ON GIRSIUM SETOSUM COMPLEX
    Sun Zhi-ying, Li Fa-zeng
    Bulletin of Botanical Research    1999, 19 (2): 23-27.  
    Abstract196)      PDF(pc) (193KB)(163)       Save
    The paper introduces the morphological project on Cirsium setosum (Willd.) MB. and C. segetum Bge. It is analysed for the 200 C. setosum and C. segetum specimens from 20 provinces of China by means of pictorialized scatter diagram and histogram, and the result support the idea that C. setosum and C. segetum are both two independent species.
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    A STUDY ON ISOZYMES OF ESTERASE OF GENUS AMARANTHUS IN CHINA
    Fan Shou-jin, Zhao Zun-tian
    Bulletin of Botanical Research    1999, 19 (2): 28-32.  
    Abstract304)      PDF(pc) (205KB)(196)       Save
    Isozymes of esterase of 13 species in genus Amaranthus were analyzed by using polycrylamide gel electrophoresis. As a result, esterase isozyme patterns show 2 representitive bands, and we think Amaranthus is a natural classification group. The taxonomic significance of esterase isozymes were discussed based on the Rf values and the similarity values of isozyme patterns. In the cultivated forms A. paniculatus, A. caudatus and A. hypochondriacus have a certain relationship. According to the relationship of isozyme product and evolution we consider section Amaranthus in more evolutionary than section Blitopsis.
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    PRELIMINARY STUDY ON NITROGENOUS METABOLISM OF GERMINATING PUCCINELLIA TENUIFLORA SEEDS UNDER ALKALINE SALT STRESS
    Li Yan-bo, Chen Yue-yan, Sun Guo-rong, Yan Xiu-feng
    Bulletin of Botanical Research    1999, 19 (2): 33-38.  
    Abstract211)      PDF(pc) (236KB)(271)       Save
    Soluble protein, free animo acid and free proline content of germinating Puccinellia tenuiflora seeds under different concentration of Na 2CO 3 stress was studied. The results indicates: in the salt free condition, soluble protein and free amino acid content increased gradually along with the germination process, proline content increased rapidly in earlier period and then keep stable; soluble protein and free amino acid content decreased under Na 2CO 3 stress compared with that in Na 2CO 3 free condition and had significant negative correlation with Na 2CO 3 concentration. Proline content showed a different tendency with total free amino and increased on different level under different concentration of Na 2CO 3 stress compared with that in Na 2CO 3 free condition.
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    STUDY ON THE DORMANCY LAW OF SEEDS OF AMBROSIA ARTEMISIFORIA AND AMBROSIA TRIFIDA
    Wang Zhi-xi, Liu Xiang-jun, Gao yi-ke, Zu Yuan-gang
    Bulletin of Botanical Research    1999, 19 (2): 39-44.  
    Abstract269)      PDF(pc) (256KB)(210)       Save
    As the annual plants of Ambrosia,Compositae, the dormancy degree of the seeds of Ambrosia artemisiforia and A. trifida varies with the latitude of growing space. The higher latitude, the more proportion of the seeds that lies dormant after maturation and the longer time of cold stratification that required for relieving the dormancy. In Mudanjiang where the latitude is higher, the mature seeds of A. artemisiforia and A. trifida lie dormant wholly and the germination percentage goes beyond 95% after twelve weeks of cold stratification, however, in Nanchang where the latitude is lower, about 10% of seeds of A. artemisiforia and A. trifida don't lie dormant and the germination percentage can go beyond 95% after eight weeks of cold stratification. Part of the dormant seeds can relieve dormancy after being stored at the room-temperature above 15℃ for half a year though they are not treated with cold stratification. The dormancy mechanism perhaps is controlled by the complex of inhibitory substance and accelerative substance. The dormant seeds contain high level of inhibitor. But for the non-dormant seeds, the balance turns favourable to accelevator. The temperature difference in different latitude, cold stratification and the course of respiratory metabolism under long-term storage at room-temperature perhaps have effects on this balance and thus change the dormancy state of seeds.
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    STUDY ON CHARACTERISTICS OF PHYSIOLOGICAL ECOLOGY OF LARIX OLGENSIS HENRY FROM DIFFERENT GEOGRAPHICAL PROVENANCES
    Wang Li-hua, Jiang Chun-ling, Feng Yu-long
    Bulletin of Botanical Research    1999, 19 (2): 45-51.  
    Abstract193)      PDF(pc) (277KB)(208)       Save
    Physiological ecology parameters of photosynthesis and water of two-year seedling obtained from hardwood cutting of Larix olgensis from ten provenances were measured in artificial climate romm. The result showed that, some rules can be found though physiological ecology parameters of Larix olgensis from different provenances varied greatly. Total photosynthesis rate> maximal net photosynthesis rate> net photosynthesis rate> net photosynthesis rate at semi-light saturation point> light respiration rate> respiration rate among all provenances, but occasional exception existed. Among all parameters, respiration rate varied least, light respiration rate varied greatly, and total photosynthesis rate varied most. The change tendency of all parameters among the provenances is in accordance with net photosynthesis rate, i. e., the other parameters varied greatly while net photosynthesis rate varied greatly and the other parameters varied less while net photosynthesis rate varied less in one provenance. Relative hight respiration rate among the provenances varied greatly, and CO 2 released amounted to one third of CO 2 fixed. Light compensation point, light saturation point and semi-light saturation among all provenances varied greatly. The more variation of water utilization efficiency resulted from net photosynthesis rate and related slightly to transpiration rate. Percentage of net photosynthesis to total photosynthesis, respiration efficiency, CO 2 compensation point, transpiration rate and water potential varied slightly among all provenances.
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    TISSUE CULTURE AND ANTIHEPATITIS CONSTITUENT IN CALLI OF SWERTIA FRANCHETIANA H. SMITH
    Xiang Feng-ning, Xing Mei-qing, Xia Guang-min, Hu Feng-zu, Li Yi
    Bulletin of Botanical Research    1999, 19 (2): 52-58.  
    Abstract204)      PDF(pc) (304KB)(226)       Save
    Calli and the regenerative plants were induced from young leaves, immature seeds and hypocotyl of Swertia franchetiana H. Smith. Three kinds of media, MS, B 5 and N 6 were set up in the experiment. Calli were established with the highest frequency onto MS medeium containing 3mg/L 2,4-D and 0.5mg/L 6BA. The regenerative plants are obtained with the highest frequency onto MS medeium, Containing 0.5mg/L 6-BA and 0.2mg/L NAA. The induction and maintenence and differentiation of the calli were affected markly by the basic media and compound 2,4-D and 6-BA in the media etc. The Oleanolic acid content in calli of Swertia franchetiana H. Smith were determined by HPLC method.
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    STUDY ON THE CHEMICAL COMPOSITION OF VOLATILE OIL FROM SEEDS OF CAMPTOTHECA ACUMINATA
    Yu Tao, Wang Yang, Yin Li-jun, Zu Yuan-gang
    Bulletin of Botanical Research    1999, 19 (2): 59-62.  
    Abstract212)      PDF(pc) (173KB)(194)       Save
    The volatile oil was obtained from seeds of by steam distillation. The average yield was 0.35%. The volatile oil was analyzed by GC/MS, and fifty-five compounds, which occupied 83.74% of the total oil, were identified. The principal compositions of the volatile oil were: Trans-linaloloxide (8.94%), Phenylethyl alcohol (8.81%), Cis-linaloloxide (7.11%), Germacrene D (5.16%) and β-Bisabolene (3.52%).
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    EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS
    Zhang Heng-qing, An Li-jia, Zu Yuan-gang
    Bulletin of Botanical Research    1999, 19 (2): 63-68.  
    Abstract226)      PDF(pc) (225KB)(246)       Save
    The PCR reaction system by Promega was employed. The DNA templates of Pinus koraiensis extracted from young needles were amplified in RAPD. The effect of content of Mg 2+, dNTP, DNA templates, primers and DNA polymorase on experimental results were tested and the optimal reaction system of RAPD for korean pine was determined.
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    GENETIC DIVERSITY AND POPULATION DIFFERENTIATION OF RHODIOLA ANGUSTA
    Yan Ting-fen, Zhou Fu-jun, Yan Xiu-feng, Zu Yuan-gang
    Bulletin of Botanical Research    1999, 19 (2): 69-74.  
    Abstract210)      PDF(pc) (251KB)(192)       Save
    Genetic diversity and population differentiation of Rhodiola angusta were studied in four populations in Changbai Mountain in Jilin province and one population in Heilongjiang province using the method of horizontal starch gel electrophoresis. Allozyme data for 12 loci of 10 enzymes indicated level of genetic variability with P=0.467, A=1.48 and He=0.195, higher than Rh. sachalinensis which share the same distribution. The level of genetic variability of the four populations in Changbai Mountain increasing as the altitude rising.It provided genetic basis for adapting the habitat of high altitude and low temperature, at the same time the method of sexual reproduction accompanied by asexual reproduction ensured the number of populations. There were higher differentiation(G ST=0.134) between Pingdingshan in Heilongjiang province and the populations in Changbai Mountain, and lower between populations of Changbai Mountain (G ST=0.091).
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    STUDY ON INFLUENCING FACTORS OF RAPD AND OPTIMIZATION OF RAPD EXPERIMENT CONDITIONS
    Xia Ming, Luan Fei-shi, Li Jing-peng
    Bulletin of Botanical Research    1999, 19 (2): 75-80.  
    Abstract284)      PDF(pc) (274KB)(260)       Save
    The influencing factors of RAPD were studied and the experimental parameters were optimized using Korean Pine ( Pinus koraiensis) and Mongolian Oak( Quercus mongolica). The optimal experiment conditions are as follow: 20 ul system containing 10~80ng template,150~300mmol/L dNTP, 15ng arbitary primer, 1~2 units Taq DNA polymerase. With a M.J. Thermal Cycler, optimal amplification program is 40 cycles of 1 min at 94℃, 1 min at 35~37℃, 1 min at 72℃, using block control style. A high reproducibility was obtained with the optimized experiment conditions.
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    A PRIMARYLY DISSCUSS ON THE ADAPTIVE MECHANISM AT DIFFERENT ALTITUDE LEVEL OF RHODIOLA SACHALINENSIS POPULATION
    Yan Ting-fen, Yan Xiu-feng, Zu Yuan-gang
    Bulletin of Botanical Research    1999, 19 (2): 81-86.  
    Abstract304)      PDF(pc) (242KB)(255)       Save
    Genetic diversity of Rhodiola sachalinensis was studied at the four different altitude levels in Changbai Mountain using the method of horizontal starch gel electrophoresis and the adaptive mechanism was discussed, combined with morphology and ecological factors. The studying results indicated that the mechanism of Rh. sachalinensis adaption to the environment of tundra not only reflected on the variation of morphology but on the basis of it's genetic background.
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    STUDIES ON THE POPULATION DIFFERENTIATION OF ARALIA ELATA (Miq.) SEEM. (Ⅱ) CLUSTER ANALYSIS ON THE CHEMICAL CONSTITUENTS
    Ye Wan-hui, Kuang Hai-xue, Wang Zhen-yue, Ma Quan
    Bulletin of Botanical Research    1999, 19 (2): 87-92.  
    Abstract219)      PDF(pc) (226KB)(129)       Save
    The chemical constituents of leaves of Aralia elata, collected from Xiaoxingan Mountains, Zhangguangcai Mountains and Wandashan Mountains of Heilongjiang province, had been analyzed by TLC. The chemical constituents varied among populations. The differentiation is in accordance with the variation of the key taxonomic characters. With the cluster analysis method on the chemical constituents, the specimens can be divided into 2 taxa, one is Aralia elata var. elata, the other is a new combination variety, Aralia elata var. mandshurica (Rupr. et Maxim.) W. H. Ye.
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    THE STUDY ON ECOLOGICAL NICHE OVERLAP RELATIONS OF SEVERAL POPULATIONS ON LEYMUS COMMUNITY IN NORTHEAST PLAIN
    Cong Pei-tong, Yan Ting-fen, Zhou Fu-jun, Liu Xing-hua, Zu Yuan-gang
    Bulletin of Botanical Research    1999, 19 (2): 93-99.  
    Abstract214)      PDF(pc) (270KB)(248)       Save
    In this article, ecological niche overlap of several populations of different leymus communities in Northeast leymus plain was studied and revealed utilization capability of several populations of different leymus communities on environmental resources and adaptability, spatial position capability on ecological factors. There are some numerical describes about species concurrent and competitive strategy of several populations. The research result reappeared current distribution structure and finitude environmental utilization of several populations by ecological niche and put forward rationalization proposal of improvement leymus plain.
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    ANALYSIS ON THE DYNAMIC OF ABOVEGROUND BIOMASS INCREMENT AND TIME SEQUENCE OF DIFFERENT DEYEUXIA ANGUSTIFOLIA POPULATIONS
    Ni Hong-wei, Gao Yi-ke, Nie Jiang-li
    Bulletin of Botanical Research    1999, 19 (2): 100-106.  
    Abstract315)      PDF(pc) (257KB)(118)       Save
    D. angustifoia is the dominant species in the typical meadow, marsh meadow and marsh communities of Sangjinag plain. Acccording to these, in this paper we researched the dynamic of aboveground biomass and time sequence in D. angustifoia. The result reveals that: the seasonal dynamic of density in marsh was "W" curve; the seasonal dynamic of density in typical meadow and marsh meadow was bimodal curve; the accumulation biomass was monocurve. In whole growing season, dry-matter has been accumulated. The changing law of seasonal dynamic of aboveground biomass has been revealed by time sequence.
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    STUDY ON GROWTH RHYTHM OF JUNIPERUS CHINENSIS (L.) ANT. CV. HARBINENSIS
    Li Chang-hai, Wang Xiao-dong, Gu Shu-fen
    Bulletin of Botanical Research    1999, 19 (2): 107-111.  
    Abstract248)      PDF(pc) (190KB)(137)       Save
    In this article we have comparatively analysed the growth rhythm of Juniperus chinensis (L.) Ant. cv. harbinensis with mathematical model and diagram, revealed the growth rhythm of Juniperus chinensis (L.) Ant. cv. harbinensis.
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    CANOPY DISTURBANCE REGIMES AND GAP REGENERATION IN A KORENA PINE-BROADLEAVED FOREST IN JIAOHE,NORTHEAST CHINA
    Zang Run-guo, Xu Hua-cheng
    Bulletin of Botanical Research    1999, 19 (2): 112-120.  
    Abstract314)      PDF(pc) (349KB)(383)       Save
    This paper deals with the canopy disturbance regimes and gap regeneration in a Korean pine-broadleaved forest in the nature reserve of Jiaohe Forest Farm, northeast China. The results showed that the percentages of expanded and canopy gaps in the forest landscape is 18.09% and 12.51% respectively. The return interval of canopy disturbance is about 700a. The average size of CG is about 69% of EG size. The range of EG size is 17~284m 2, with an average of 75.49m 2. The range of CG size is 10~234m 2, with an average of 51.98m 2. The average diameter of most gaps are about 20-60% of the average height of canopy trees. Most gaps were formed by one gap maker, and the most important manners of gap formation were breakage at trunk base and uprooting. Major gap makers in the forest were Pinus koraiensis, Abies holopylla, Betula costata and Picea jezoensis. The DBH of most gap makers were 40~80cm, and their heights 25~30m. The distribution pattern of gaps in the forest landscape belongs to the uniform type. The quantitative characteristics of different tree species in gaps and non-gap stands are different. With the alternation of gaps and non-gap stands, the relative dominance of different species changed correspondently. Tree species were classified into three regeneration response types according to the difference of orders of importance values in gaps and in non-gap stands. Diversity characteristics of species were also different in gaps and non-gap stands.
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    A NEW SPECIES OF SCLERIA FROM YUNNAN
    Qian Yi-yong
    Bulletin of Botanical Research    1999, 19 (3): 241-243.  
    Abstract203)      PDF(pc) (136KB)(155)       Save
    Scleria jiangchengensis Y. Y. Qian, sp. nov. (Subgen, Scleria, Sect. Elatae)
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    NOTES ON DROSERA ROTUNDIFOLIA LINN. VAR. FURCATA Y. Z. RUAN
    Lin Qi
    Bulletin of Botanical Research    1999, 19 (3): 244-245.  
    Abstract250)      PDF(pc) (86KB)(189)       Save
    Based on the study of Drosera rotundifolia Linn. specimens from Asia, America and Europe, Drosera rotundifolia Linn. var. furcata Y. Z. Ruan is regarded as a new synonym of D. rotundifolia Linn.
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