Top Read Articles

Published in last 1 year |  In last 2 years |  In last 3 years |  All

Please wait a minute...


For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails

Select

Analysis of Metabolic Characteristics of Different Organs of Acanthopanax senticosus and Acanthopanax sessiliflorus Based on GC-MS Yanjun REN, Yu SUN, Xiaorui GUO, Mingyuan XU, Wenda SHAO, Kexin WU Bulletin of Botanical Research    2023, 43 (3): 439-446.   DOI: 10.7525/j.issn.1673-5102.2023.03.014 Abstract （3145）   HTML （8）    PDF（pc） （1430KB）（74）       Knowledge map    Save To clarify the differences of primary metabolism between Acanthopanax senticosus and A. sessiliflorus in different organs， and to explore their pharmacodynamic differences， medicinal value， and resource utilization structure， the metabolomics research strategy and GC-MS technology were used as methods. The results showed that 186 primary metabolites were identified in different parts of the two medicinal plants， and there were differences in primary metabolism between the two species. Further analysis showed that 52 differential metabolites in roots， 34 differential metabolites in stems， 39 differential metabolites in leaves， and 48 differential metabolites in petioles， were obtained respectively. The main metabolic pathways involved in differential metabolites were the aminoacyl tRNA biosynthesis pathway； biosynthetic pathways of valine， leucine， and isoleucine； glycine， serine， and threonine metabolic pathways； biosynthetic pathway of arginine； alanine， aspartate， and glutamate metabolic pathways； pyruvate metabolic pathway， respectively. In addition， there were differences in the levels of four key metabolites connecting primary metabolism and secondary metabolism in different organs respectively. A. senticosus and A. sessiliflorus were significantly different in the primary metabolism of different organs， which might be one of the reasons for the differences in pharmacological effects and secondary metabolism of different organs between the two species. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics

Select

Cloning of WRKY40 Gene from Leymus chinensis and Analysis of Disease Resistance in Transgenic Tobacco Danni LI, Jiali LIU, Jitao ZHANG, Baoxiang GU, Fengjin ZHU, Qingjie GUAN Bulletin of Botanical Research    2023, 43 (3): 412-420.   DOI: 10.7525/j.issn.1673-5102.2023.03.011 Abstract （3134）   HTML （9）    PDF（pc） （3682KB）（90）       Knowledge map    Save In order to cultivate rice varieties resistant to Xanthomonas oryzae pv. oryzae， Rhizoctonia solani， Magnaporthe oryzae and Fusarium fujikuroi， the method of mining resistance genes is an important way to select resistant varieties. RT-PCR method was used to clone the LcWRKY40 gene （MN187915） from the leaves of Leymus chinensis. The result showed that the CDS was 1 053 bp in length and encoded 350 amino acids with a molecular weight of 38.1 kDa. The results of bioinformatics analysis showed that the primary structure of LcWRKY40 contained WRKY domain， zinc finger protein domain and nuclear localization sequence. Phylogenetic tree construction and motif analysis showed that the phylogenetic relationship between LcWRKY40 and HvWRKY38 was closed. The results of subcellular localization in tobacco showed that the LcWRKY40 protein was located in the nucleus， verified by the software prediction. qRT-PCR tissue specific expression analysis showed that LcWRKY40 gene was expressed in root， stem， leaf， leaf sheath， Lemma and anther of Leymus chinensis respectively， but the expression level was the highest in leaf but the lowest in Lemma. Transgenic LcWRKY40 tobacco plants and wild-type tobacco plants were inoculated with X. oryzae pv. Oryzae， R. solani， M. oryzae and F. fujikuroi， respectively， which showed that transgenic LcWRKY40 tobacco plants could alleviate the four pathogens in different degrees， and showed high resistance to M. oryzae and F. fujikuroi. Therefore， it was speculated that LcWRKY40 protein played a key regulatory role in signal pathways such as resisting disease stress and improved the resistance of plant pathogens， which layed a molecular foundation for the study of LcWRKY40 function and abiotic stress. Table and Figures | Reference | Related Articles | Metrics

Select

Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica Chunjing JIAO, Mingyue LI, Peng ZHANG Bulletin of Botanical Research    2023, 43 (3): 370-378.   DOI: 10.7525/j.issn.1673-5102.2023.03.006 Abstract （3125）   HTML （9）    PDF（pc） （1563KB）（112）       Knowledge map    Save To explore the effects of exogenous hormones soaking combined with polyethylene glycol 6000 （PEG） osmotic treatment on the thermal dormancy and related physiological changes in seeds of Fraxinus mandshurica， non-dormant F. mandshurica seeds were used as materials and then soaked with 1 mmol·L-1 gibberellin（GA4+7）， ethylene（ETH） and cytokinins（6-BA） for 24 h respectively， and the germination of seeds cultured in different mass fractions（0， 5%， 25%， 50%） PEG solution at the induction thermal dormancy temperature（25 ℃）for 7 d and then at 10 ℃ was assayed. On this basis， the treatments with significant results were selected to determine the changes in seeds endogenous hormones content and cell wall degrading enzyme activity. The results showed that： compared with hormone treatment alone， GA4+7 combined with different mass fraction PEG had no significant effect on germination ability. With the increase of PEG mass fraction， the germination ability of 6-BA combined with PEG treatment gradually increased and then tend to stabilized， when combined with 25% PEG， the germination percentage and germination index respectively increased 69.31% and 1.04， and the germination time was shortened by 7.8 d. The seeds germination ability treated ETH combined with PEG gradually increased with the increase of PEG mass fraction， when combined with 50% PEG， the germination percentage and germination index respectively increased 43.43% and 0.76. Hormone combined with osmotic treatment significantly increased endosperm cellulase activity， but the GA and ABA contents in embryo and endosperm were not significantly regularity. In conclusion， exogenous hormone combined with osmotic treatment could reduce the thermal dormancy of F. mandshurica seeds， and the effect was different depending on the hormone species. Table and Figures | Reference | Related Articles | Metrics

Select

Genetic Transformation and Function Analysis of PsnHB13 and PsnHB15 of Populus simonii × Populus nigra Zhanmin ZHENG, Yubing SHANG, Guangbo ZHOU, Di XIAO, Yi LIU, Xiangling YOU Bulletin of Botanical Research    2023, 43 (3): 340-350.   DOI: 10.7525/j.issn.1673-5102.2023.03.003 Abstract （3118）   HTML （10）    PDF（pc） （3156KB）（72）       Knowledge map    Save Transcriptome analysis in Populus simonii × P. nigra overexpressing PsnCycD1；1 showed PsnHB3 and PsnHB15 are differential genes. Analysis of PsnHB13 and PsnHB15 conservative domains through InterPro tool. The STRING software was utilized to explore PsnHB13 and PsnHB15 proteins interaction network analysis. Yeast two hybrid was used to verify the interaction proteins，calculate the ratio of leaf length to width，the ratio of dry weight to fresh weight of leaves and stems of transgenic plants，and analyze the transcriptome of PsnHB13 overexpression plants. Conserved domain analysis showed that PsnHB13 and PsnHB15 genes belonged two subfamilies，showing that PsnHB13 mainly contains the HDZip I domain，and PsnHB15 mainly contains the HDZip Ⅲ domain. ，Each gene is screened to 10 interacting genes. Moreover，PsnHB15 has a higher probability of interaction with family proteins. Yeast two-hybrid assays demonstrated that PsnHB15 interacts with PsnHB13 and PsnHB13 interacts with PsnCycD1；1. The aspect ratio of leaves in transgenic lines overexpressing PsnHB13 and PsnHB15 increased in the early seedling stage.The ratio of dry weight to fresh weight in stems of PsnHB13 overexpression lines increased significantly（P<0.05）. The analysis of the transcriptome revealed that Gene Ontologies（GO） enrichment analysis identified 3 significantly enriched GO terms，including response to chemical，response to organic substance，regulation of RNA metabolic process. KEGG pathway enrichment analysis demonstrated that transcription factors，plant hormone signal transduction and Cytochrome P450 were significantly enriched. These differential genes，including MADS-box transcription factor，MYBP transcription factor，ERF1 transcription factor，GH3 auxin responsive genes，SAUR protein family. PsnHB13 and PsnHB15 play an important role in the growth and development of Populus simonii × P. nigra，and are key genes to explore the growth law of plants and reveal the relationship between cell cycle and growth regulation. Table and Figures | Reference | Related Articles | Metrics

Select

Analysis on Leaf Color and Growth Variation of Transgenic BpGLK Betula pendula ‘Dalecarlica' Li CAO, Yunli YANG, Tianfang LI, Jing JIANG Bulletin of Botanical Research    2023, 43 (3): 351-360.   DOI: 10.7525/j.issn.1673-5102.2023.03.004 Abstract （3117）   HTML （8）    PDF（pc） （1713KB）（114）       Knowledge map    Save Table and Figures | Reference | Related Articles | Metrics

Select

The Pollination Biology of Begonia Guangxiensis, a Narrowly Distributed Species Qiongyue LIANG, Changqiu LIU, Qifeng LU, Tao DENG, Bo CAI Bulletin of Botanical Research    2023, 43 (3): 432-438.   DOI: 10.7525/j.issn.1673-5102.2023.03.013 Abstract （3107）   HTML （7）    PDF（pc） （1497KB）（88）       Knowledge map    Save To examine the mating system of Begonia guangxiensis and whether the species relies on particular pollinators in its native habitat for pollination and whether it can achieve sexual reproduction in artificial habitats， the experiment of floral longevity observation， floral traits determination， pollination， flower visitor observation and seed germination determination of B. guangxiensis were carried out in this study. The result showed that B. guangxiensis had a long floral longevity， and the corolla sizes varied in different habitats； there was no significant difference in the proportion fruit set and seed germination rate between self-pollination and cross-pollination treatments and natural control between the two habitats； flower visitors included bees（Apis cerana， Bombus eximius） and hoverflies（Episyrphus balteatus， Ischiodon scutellaris）， and the visitor composition varied with habitats and years. All the results consistently indicated that the self-compatible B. guangxiensis relied on pollinators for sexual reproduction， but did not rely on a particular type of pollinator， but had a more generalized pollination system， and therefore could reproduce successfully in unnatural habitats. Table and Figures | Reference | Related Articles | Metrics

Select

Selection of Suitable Mosses for Bare Rock and Steep Slope Greening after Jiuzhaigou Earthquake Based on the Analytic Hierarchy Process Hongxia XIA, Lilan LIU, Xuping ZHOU, Ke CHEN, Ying LI, Peigang KUANG, Geng SUN, Yanbao LEI Bulletin of Botanical Research    2023, 43 (4): 540-549.   DOI: 10.7525/j.issn.1673-5102.2023.04.007 Abstract （2688）   HTML （6）    PDF（pc） （1411KB）（1426）       Knowledge map    Save In order to promote the rapid greening of the steep bare rock slopes in the Jiuzhaigou National Nature Reserve after the earthquake， and accelerate the ecological restoration process of the landscape， based on references and field investigations， 30 common native mosses in the region were selected， and an evaluation index system of 18 items was constructed based on six aspects including resistance ability， availability， ecological function， reproductive ability， biomass accumulation， and aesthetic appeal， using the Analytic Hierarchy Process（AHP）. The results indicated that： according to the comprehensive evaluation score， the mosses were divided into four grades. Finally， five species， Racomitrium japonicum， Hypnum plumaeforme， Eurohypnum leptothollum， Plagiomnium ellipticum and Brachythecium rutabulum were selected. The results should provide an objective guidance for future ecological restoration without soil in Jiuzhaigou and its surrounding cold fragile ecosystem area. Table and Figures | Reference | Related Articles | Metrics

Select

Regulation of Salicylic Acid on Tolerance to Saline Alkali Stress at Seedling Stages of Betula platyphylla Jingzhe WANG, Chaokui NIU, Xinyuan LIANG, Chenjing SHEN, Jing YIN Bulletin of Botanical Research    2023, 43 (3): 379-387.   DOI: 10.7525/j.issn.1673-5102.2023.03.007 Abstract （2067）   HTML （11）    PDF（pc） （1371KB）（88）       Knowledge map    Save To investigate the effects of salicylic acid（SA） on growth and secondary production synthesis of Betula platyphylla seedling under saline alkali stress， three treatments were applied to birch seedlings： water treatment（control）， 200 mmol·L-1 NaHCO3， 200 mmol·L-1 NaHCO3+360 μmol·L-1 SA. The relative conductivity， chlorophyll fluorescence parameters， osmotic content， anti-stress enzyme activity， the relative expression of key genes of triterpene synthesis pathway in the middle leaves of birch seedlings in response to different treatments were detected respectively， and the total triterpenoids， flavonoids and polyphenols of birch seedlings were analyzed. The results showed that： ①under the 200 mmol·L-1 NaHCO3 treatment， then 360 μmol·L-1 SA treatment significantly reduced the relative conductivity of the leaves of birch seedlings， improved the Fv/Fm value of chlorophyll fluorescence parameters of birch seedlings， increased the content of the osmotic regulator proline and soluble protein， and enhanced the activities of antioxidant enzymes（SOD， POD， CAT， APX） in birch seedlings to a certain extent. ②200 mmol·L-1 NaHCO3 saline alkali stress promoted the upregulation of the expression of key genes of triterpene synthesis pathway FPS， SS， SE， BPX and BPW in birch seedlings， and significantly promoted the synthesis of polyphenols in birch seedlings in the early stage， and significantly promoted the accumulation of total triterpenoids and flavonoids in the late stage of saline-alkali stress（P<0.05）. The application of SA under saline-alkali stress promoted the upregulation of HMGR， FPS， SE， and BPX gene expression in birch seedlings， and promoted the synthesis of flavonoids in the early stage of stress， and significantly promoted the accumulation of total triterpenes in the late stage of stress， which was 34% higher than that of the saline-alkali stress group and 47% higher than that of the water treatment on 7th day. Overall， under saline-alkali stress， SA with suitable concentration improves the tolerance to saline-alkali of birch seedlings by adjusting osmotic equilibrium， alleviating light inhibition， enhancing the antioxidant defense system of birch seedlings， and regulating substance metabolism. Table and Figures | Reference | Related Articles | Metrics

Select

Anatomical Structure and Ecological Adaptability of Stems and Leaves of Rhamnus ussuriensis Qingsong WU, Yinghui LIU, Shuo LI, Panpan LI, Youmin ZHANG Bulletin of Botanical Research    2023, 43 (3): 461-469.   DOI: 10.7525/j.issn.1673-5102.2023.03.016 Abstract （2045）   HTML （7）    PDF（pc） （8959KB）（127）       Knowledge map    Save To investigate the adaptability mechanism of the anatomical structure of the Rhamnus ussuriensis stems and leaves to the environment， the stems and leaves of Rh. ussuriensisis were studied by paraffin sections and scanning electron microscopy. The results showed that： leaf blade of Rh. ussuriensisis was bifacial； trichomes and stomata were distributed in lower epidermis； stomatal index was 39.04 %； the palisade tissue was composed of two layers of parenchyma cells； the palisade tissue had a compact structure， however， the spongy tissue was loosely arranged. The main veins of the leaves were developed， the vascular bundles were arranged in the ring-shape， the number of xylem vessels and the diameter was large respectively， and the parenchyma cells around the vascular bundle contained abundant ergastic substance. In the primary structure of stem， the cuticle of epidermal cells was thick， and the cells of cortex parenchyma contained crystal clusters. The vascular bundle was open collateral vascular bundle； the medulla was developed； the secondary structure of the stem had obvious annual rings， which was a typical ring-porous wood， with concave lenticels and developed secondary xylem； most of the vessels were spiral vessels and pitted vessels， and the vessel member were mostly compound pores. The rays were mainly single rays， with a small number of double rays. The anatomical structure of the R. ussuriensishas leaves and stems had obvious characteristics of drought resistance and cold resistance， it might adapt to arid and cold environments. Table and Figures | Reference | Related Articles | Metrics

Select

The Function of Salt and Alkaline Tolerance of WRKY42 Gene in Amorpha fruticosa Yu SUN, Yiteng ZHANG, Huihui CHENG Bulletin of Botanical Research    2023, 43 (4): 612-621.   DOI: 10.7525/j.issn.1673-5102.2023.04.014 Abstract （1992）   HTML （11）    PDF（pc） （3957KB）（1484）       Knowledge map    Save To investigate the role of WRKY transcription factors（TFs） in sensing saline-alkali stress signals and maintaining their tolerance function through physiological and biochemical regulatory pathways， and the WRKY42 gene of Amorpha fruticosa was cloned and the expression pattern in response to salt（NaCl） and alkali（NaHCO3） stress and tissue organs was analyzed， and the salinity tolerance function was also studied its by overexpression in tobacco. In this study， the AfWRKY42 gene was cloned based on transcriptome sequencing data of A. fruticosa L. under stress. Bioinformatics analysis showed that AfWRKY42 contained a WRKY structural domain， two low-complexity regions and a helix region. Phylogenetic tree analysis of amino acids revealed that AfWRKY42 was most closely related to WRKY47 of Cajanus cajan and WRKY42 of Mucuna pruriens. The localization of AfWRKY42 protein in mesophyll protoplasts of Arabidopsis thaliana was confirmed in nucleus by a transient gene expression system. Quantitative analysis of AfWRKY42 gene showed the highest expression in the shoot epidermis of Sophora japonica. Detection of expression patterns in roots and leaves treated with NaCl and NaHCO3 showed an overall increasing trend induced by it， suggesting that overall stress induced an increase in AfWRKY42 gene expression， and AfWRKY42 gene was associated with the regulation of salinity tolerance in plants. Analysis of salinity tolerance in 35S-initiated overexpressing T3 generation of tobacco lines transgenic for AfWRKY42 gene showed that the transgenic tobacco lines showed increased resistance after salinity stress treatment， it had higher chlorophyll and electrical conductivity and significantly lower malondialdehyde content than the wild type， indicating that AfWRKY42 played an important regulatory role in response to salinity stress. It would provide a WRKY transcription factor candidate gene for salinity resistance breeding and lay the foundation for improving the resistance of A. fruticosa and other plants. Table and Figures | Reference | Related Articles | Metrics

Select

Infraspecific taxa of Paris fargesii Franch. Zixuan REN, Ling ZHAO, Jiawen ZHAO, Qinghe WANG, Hongxiang YIN Bulletin of Botanical Research    2023, 43 (4): 505-519.   DOI: 10.7525/j.issn.1673-5102.2023.04.004 Abstract （1862）   HTML （12）    PDF（pc） （7354KB）（1813）       Knowledge map    Save In order to explore the taxonomic composition of Paris fargesii Franch.， the morphological characteristics， phenological period， phytochemistry， molecular biology and geographical distribution of P. fargesii Franch. were compared and analyzed respectively. The results showed that there were five taxa， including P. fargesii var. fargesii， P. fargesii var. latipetala， P. fargesii var. brevipetalata， P. fargesii var. macrosepala and the black patch population. All of them differed in morphology， phenological period， steroidal saponins composition and ITS sequence， and had their own geographical distribution range. The phenological period and chemical composition of P. fargesii var. petiolata were closer to P. delavayi， and clustered into one branch in the ITS phylogenetic tree， and there was a transitional group between them. Therefore， P. fargesii var. petiolata should be removed from P. fargesii， as a variety of P. delavayi， which scientific name was P. delavayi var. petiolata. It was speculated that the infraspecific evolution order of P. fargesii was P. fargesii var. brevipetalata→P. fargesii var. latipetala→P. fargesii var. fargesii→P. fargesii var. macrosepala， black patch population. Table and Figures | Reference | Supplementary Material | Related Articles | Metrics

Select

Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Schisandra chinensis and the Effects on Anti-Oxidative Stress in Cells Hanlin ZHU, Heng ZHAO, Bowen ZHAI, Maoyu ZHANG, Yujie FU Bulletin of Botanical Research    2023, 43 (4): 631-640.   DOI: 10.7525/j.issn.1673-5102.2023.04.016 Abstract （1858）   HTML （13）    PDF（pc） （3235KB）（1643）       Knowledge map    Save The polysaccharides of Schisandra chinensis were extracted by ultrasonic-assisted cellulase enzymolysis from the dried fruit of S. chinensis as raw material， and the extraction process and anti-oxidative stress activity of S. chinensis polysaccharides were studied. Using polysaccharide yield as index， single factor experiment and Box-Behnken response surface test were used to investigate the effects of enzyme concentration， ultrasonic power， extraction time and solid-liquid ratio on polysaccharide yield of S. chinensis， and the ultrasonic-assisted enzymatic extraction process was optimized. A lipopolysaccharide（LPS） induced oxidative stress damage model of HepG2 cells was established， and the levels of malondialdehyde（MDA）， superoxide dismutase（SOD） and reactive oxygen species（ROS） were used as indicators， and the anti-oxidative stress ability of S. chinensis polysaccharides was investigated. The results showed that the optimal extraction conditions as followed： the enzyme concentration was 1 420 U·g-1， the ultrasonic power was 500 W， the extraction time was 46 min， the solid-liquid ratio was 1 g∶32 mL. Under these conditions， the yield of polysaccharides was 22.25%. Moreover， the extracted S. chinensis polysaccharides might improve the SOD level in the LPS-induced HepG2 cells， but reduce the MDA accumulation and ROS fluorescence intensity in the cells， and effectively relieve the LPS-induced oxidative stress in HepG2 cells. The extraction conditions of polysaccharides from S. chinensis were optimized， and it was found that the extracted polysaccharides had good antioxidant stress ability. Table and Figures | Reference | Related Articles | Metrics

Select

Verification of Scientific Name of Stauranthera grandifolia Benth. Zhangjie HUANG, Peiliang LIU Bulletin of Botanical Research    2023, 43 (4): 493-497.   DOI: 10.7525/j.issn.1673-5102.2023.04.002 Abstract （1849）   HTML （21）    PDF（pc） （2840KB）（1483）       Knowledge map    Save The correct scientific name is the only international name of a species， which could reduce ambiguity in communication， but it would cause confusion in research and production if it is used incorrectly. As a hotspot plant Family， there were some problems of changes in scientific names in Gesneriaceae. Since the publication of Stauranthera grandifolia Benth.， different forms of the epithet， grandifolia and grandiflora， were used in various literatures. By checking related literatures， this paper confirms that the correct epithet should be grandifolia according to the Articles of the International Code of Nomenclature for Algae， Fungi， and Plants. Table and Figures | Reference | Related Articles | Metrics

Select

Cloning and Functional Analysis of Deubiquitinating Enzyme Gene UCHs from Hevea brasiliensis Mingyue YUAN, Tianzhong ZHOU, Ma YU, Bin HU, Xiangyu LONG, Hua CHEN Bulletin of Botanical Research    2023, 43 (4): 622-630.   DOI: 10.7525/j.issn.1673-5102.2023.04.015 Abstract （1844）   HTML （8）    PDF（pc） （1813KB）（1088）       Knowledge map    Save To investigate the potential function of ubiquitin carboxyl terminal hydrolases（UCHs） in the ubiquitination process of laticiferous of Hevea brasiliensis， the full-length sequences of two UCHs family members（HbUCH-L3 and HbUCH-L5） were isolated from Hevea brasiliensis， and both had typical UCHs domains. The open reading frame of HbUCH-L3 and HbUCH-L5 were 993 bp and 558 bp， and encoded 330 and 185 amino acids， respectively. The results of qRT-PCR showed that HbUCH-L3 and HbUCH-L5 were constitutively expressed in all tissues， but was low in latex. In vitro ubiquitination substrates cleavage of recombinant HbUCHs showed that both HbUCH-L3 and HbUCH-L5 had the function of hydrolyzing ubiquitin. HbUCHs significantly reduced the overall ubiquitination level of C-serum proteins； while， the deubiquitinating activity of HbUCH-L3 was higher than that of HbUCH-L5. Therefore， it was speculated that UCHs played a role in maintaining the dynamic balance of laticiferous ubiquitination and thus played a specific biological function， but the exact mechanism was still unclear. Table and Figures | Reference | Related Articles | Metrics

Select

Moss New Records in China from Gongga Mt., Sichuan Qinghua WANG, Qiang HE, Lian LUO, Gaowa NAREN, Zhaoqin YI, Xiaoao ZHENG, Yong JIANG, Yu JIA Bulletin of Botanical Research    2023, 43 (4): 498-504.   DOI: 10.7525/j.issn.1673-5102.2023.04.003 Abstract （1842）   HTML （18）    PDF（pc） （2719KB）（1609）       Knowledge map    Save Three moss species， including Ctenidium malacodes， Ulota crispula and Ulota intermedia， were reported as new records and collected from Gongga Mt. in Sichuan， and all specimens were deposited in the Herbarium， Institute of Botany， Chinese Academy of Sciences（PE）. We provided detailed morphological descriptions and photos and discuss their main differences from neighboring species. The bryophyte diversity in Gongga Mt. area is very high， which is worthy of further investigation and research. Table and Figures | Reference | Related Articles | Metrics

Select

Bryophyte Diversity of Underground Forests in Craters of Jingpohu Global Geopark Mingyang CONG, Yongkun LI, Wenjing YANG, Puqing CHEN Bulletin of Botanical Research    2023, 43 (3): 361-369.   DOI: 10.7525/j.issn.1673-5102.2023.03.005 Abstract （1835）   HTML （13）    PDF（pc） （1195KB）（110）       Knowledge map    Save To explore the bryophyte diversity of underground forests in craters of Jingpohu Global Geopark， the typical investigation method was used， and a total of 3 622 bryophyte specimens were collected， and morphological and anatomical methods were used to identify the specimens. The results showed that， 292 species（126 genera and 53 families） were recorded， 31 species （17 genera and 14 families） were liverworts， 261 species （109 genera and 39 families） were mosses. The number of bryophyte species of four craters was 192 species in Ⅳ， 126 species in Ⅰ， 113 species in Ⅲ， and 61 species in Ⅱ， respectively. The dominant families were Brachytheciaceae， Mniaceae， Pottiaceae， etc.， accounting for 42.12% of the total species， and the dominant genera were Brachythecium， Entodon， Plagiomnium， etc.， accounting for 18.49% of the total species， and the dominant species were Pylaisia polyantha， Pylaisiadelpha yokohamae， Entodon flavescens， respectively. There were 20 common species of bryophytes in four craters， and the unique species in the Ⅳ crater was the most（88 species）. The diversity of the ‘moderate interference hypothesis’ was supported， and the similarity of bryophytes between Jingpo Lake crater and Wudalianchi volcanos was high（20.42%）， which revealed that the terrain contributes greatly to the diversity. The alpha diversity of bryophytes in the crater I exhibited a mid-peak pattern， which was in line with the ‘Mid-elevation bulge’ theory， priority should be given to the central elevation where the bryophytes were the most abundant. The study showed that the underground forests in Jingpohu Global Geopark craters preserved high bryophyte diversity and played an important role in the volcanic ecosystem of China， and a key protection should be given to bryophytes in this volcanic geological wonder to preserve genetic resources， and the results provided first-hand basic information and important scientific significance. Table and Figures | Reference | Related Articles | Metrics

Select

Detailed Phenotypical Analysis on the Mutant ssr1- 2 Encoding a Mitochondrial Protein of Arabidopsis thaliana Yuanyuan CAI, Jibenben XIA, Wenhan YING, Jieyao WANG, Tao XIE, Kongya XING, Xuanjun FENG, Xuejun HUA Bulletin of Botanical Research    2023, 43 (3): 421-431.   DOI: 10.7525/j.issn.1673-5102.2023.03.012 Abstract （1764）   HTML （8）    PDF（pc） （5062KB）（128）       Knowledge map    Save To explore the role of SSR1 gene encoding a functionally unknown mitochondrial protein of Arabidopsis thaliana in plant growth and stress response， ssr1-2 and its suppressor mutants EMS143 and EMS145 were used to track the growth of their roots and aboveground parts， as well as their proline sensitivity and iron homeostasis， and the effects of ssr1-2 short root phenotype on aboveground growth of seedlings were analyzed by micrografting. The results showed that the primary root length of ssr1-2 was shorter and the root architecture was similar to the fibrous root system. The growth of their shoots was also retarded， but appeared considerably later than the short-root phenotype. The results of micrografting revealed that the roots of ssr1-2 could restrict the growth of the wild-type shoots and， and conversely， the shoot of ssr1-2 could also affect the growth of the wild-type roots， but the former had a bigger effect. ssr1-2 showed a hypersensitive phenotype to proline in seed germination， root length and leaf chlorophyll content. In addition， ssr1-2 appeared insensitive to Fe nutrition， that was， the stimulating effect of Fe salt on seedling growth was significantly less than that of wild-type WS， indicating a significant decrease in its ability to utilize Fe. The results suggested that SSR1 might be involved in the regulation of A. thaliana root growth by affecting the utilization of iron nutrient， implying that the impairment of the mitochondrial iron utilization machinery might contribute significantly to the enhanced inhibitory effect of proline on plant growth and development. Table and Figures | Reference | Related Articles | Metrics

Select

Pollen Morphological Characters of 20 Species from Camellia Sect. Theopsis and Sect. Eriandria Yali ZHANG, Naiying YANG, Yao SONG, Xiangpeng LI, Weizhen GUO, Youming CAI Bulletin of Botanical Research    2023, 43 (3): 470-480.   DOI: 10.7525/j.issn.1673-5102.2023.03.017 Abstract （1747）   HTML （11）    PDF（pc） （3413KB）（65）       Knowledge map    Save To provide references for the phylogenetic evolution， classification and identification of plants in Camellia Sect. Theopsis and Camellia Sect. Eriandria were studied by pollen morphology. 20 species of the two sections， pollen shape and exine sculpture were used as materials and observed by scanning electron microscopy（SEM）. Results indicated： （1）Pollen size of 20 species mainly belonged to medium（25-50 μm） and large（50-100 μm） pollen. The pollens were all single-grain pollen with three grooves， three-cracked in the polar view and long spherical in the equatorial view. （2）There were three types of pollen exine sculpture， including foveolate， granulate and verrucate. C. elongata was foveolate type， C. forrestii， and C. forrestii var. acutisepala were verrucate type， and the other 17 species were granulate type. We considered that the evolution trend of pollen exine sculpture of the two sections was foveolate→granulate→verrucate. The results of pollen exine sculpture types supported the view that Sect. Theopsis and Sect. Eriandria were a monophyletic clade， while the taxonomic status of C. tsofuii and C. lipoensis should be further studied. Table and Figures | Reference | Related Articles | Metrics

Select

Regulatory Effects of Salicylic Acid,Nano-zinc Oxide and Growth-promoting Fungi YZ13-1 on the Resistance to Drought Stress of Fraxinus mandshurica Chenjing SHEN, Wenbo WU, Luran GENG, Fulong WANG, Pengzhou ZHAO, Jinhui SONG, Yaguang ZHAN, Jing YIN Bulletin of Botanical Research    2023, 43 (3): 388-395.   DOI: 10.7525/j.issn.1673-5102.2023.03.008 Abstract （1735）   HTML （8）    PDF（pc） （1463KB）（91）       Knowledge map    Save To investigate the effects of salicylic acid（SA）， nano-zinc oxide（ZnO NPs） and growth-promoting endophytic fungus YZ13-1（Fusarium graminearum） on drought tolerance of Fraxinus mandshurica seedlings. Two years old potted F. mandshurica seedlings were used as materials and 5 groups of treatments were set in this experiment：T1，drought stress treatment （soil water content was 20%-25%）； T2， T3 and T4 groups were additionally treated with 50 mg·L-1 SA，100 mg·L-1 ZnO NPs， growth-promoting fungus YZ13-1 suspension on the basis of T1 group； CK， control group（soil water content was 50%-55%）. The results showed that compared with T1 group， the activities of peroxidase（POD）， ascorbate peroxidase（APX）， catalase（CAT） were increased by 2 times， soluble sugar（SS）， proline（PRO） and phenolic substances increased by 44%， 60%， 16%， and relative electrical conductivity（RWC） of leaves decreased by 14% in T2 group. Compared with endophytic fungi and ZnO NPs， SA could significantly increase the anti-stress enzyme activity， increase the contents of osmotic substances and phenolic substances， and thus improve the drought resistance ability of F. mandshurica under drought stress. The study clarified that three induction treatments could improve the drought resistance of F. mandshurica， and SA treatment was the best， providing a theoretical basis for screening drought resistance agents of F. mandshurica. Table and Figures | Reference | Related Articles | Metrics

Select

Effects of Cryopreservation on Physiological and Biochemical Characteristics of Fraxinus mandshurica Embryos Yue REN, Cheng WEI, Tiantian XU, Hailong SHEN, Ling YANG Bulletin of Botanical Research    2023, 43 (3): 396-403.   DOI: 10.7525/j.issn.1673-5102.2023.03.009 Abstract （1700）   HTML （6）    PDF（pc） （1479KB）（113）       Knowledge map    Save In this paper， the zygotic embryos were used as storage materials to further analyze the changes of physiological and biochemical indexes of the zygotic embryos before and after cryopreservation， and the effects of freezing method and dewatering time on physiological and biochemical indexes were clarified， which provided physiological data support for the establishment of long-term storage methods of fine genetic materials of Fraxinus mandshurica. Under the condition of dehydration for 120 min， the activity of dehydrogenase in zygote embryos was the highest， and the activity of catalase， peroxidase and mass fraction of proline reached the maximum. They were 1 168.85 U·g-1·min-1， 338.33 U·g-1·min-1 and 394.99 μg·g-1， respectively. Dehydrogenase activity， proline content， peroxidase activity and catalase activity in zygotic embryo cells were all the minimum values after vitrification. The soluble protein fraction and superoxide dismutase activity in zygote embryos reached the maximum values after storage by slow freezing method， which were 18.82 mg·g-1 and 361.97 U·g-1， respectively， which were significantly higher than those by other freezing methods. The activity of zygotic embryos reached the highest value under the conditions of drying and dewatering for 120 min and fast freezing. The results showed that the survival rate and physiological indexes of the zygotic embryos of F. mandshurica were affected by different freezing methods， dehydration time and their interaction. In addition to the decrease of malondialdehyde content， the dehydrogenase activity， antioxidant enzyme activity， proline content and soluble protein content in the zygotic embryo cells of F. mandshurica increased with the increase of dehydration time after being stored in different freezing methods. The survival rates of zygotic embryos and somatic embryos after cryopreservation were 62.26% and 51.68%. Therefore， the method of drying and dewatering for 120 min combined with fast freezing is suitable for cryopreservation of F. mandshurica embryos. Table and Figures | Reference | Related Articles | Metrics