%0 Journal Article %A FU Yu-Jie %A JIANG Shou-Gang %A WANG Zhuo %A ZHANG Yu %A ZU Yuan-Gang* %T Optimization of Extration of 7-xylosyl-10-deacetylpaclitaxel from Taxus cuspidata %D 2010 %R 10.7525/j.issn.1673-5102.2010.03.021 %J Bulletin of Botanical Research %P 369-372 %V 30 %N 3 %X In an effort to search for natural products from Taxus cuspidata with improved pharmacological features compared to paclitaxel,7-xylosyl-10-deacetylpaclitaxel has been isolated and identified by our group. The objective of the present study was to optimize and establish the extraction process of 7-xylosyl-10-deacetylpaclitaxel from T.cuspidata. RP-HPLC mothod was applied to determine the content of 7-xylosyl-10-deacetylpaclitaxel. The orthogonal test was adopted to examine the effects of the 4 factors including ethanol concentration, ethanol volume, number of extraction cycles and extraction time. Ethanol concentration and ethanol volume were essential factors influencing the extraction of 7-xylosyl-10-deacetylpaclitaxel. High concentration and amount of ethanol facilitated the extraction. Number or extraction cycles and extraction time were also important. Single-factor test indicated, 2 h and 2 cycles were the best. The optimum extractive condition are N9(A3B3C2D1) and N8(A3B2C1D3). %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2010.03.021