%0 Journal Article %A DOU Kai %A LIU Zhi-Hua* %A WANG Na %A WANG Yu-Cheng %A WANG Zhi-Ying %A ZHANG Rong-Shu %T Rooting and Transplanting Method of Populus davidiana×P.bolleana Tissue Culture Seedlings %D 2014 %R 10.7525/j.issn.1673-5102.2014.03.015 %J Bulletin of Botanical Research %P 380-385 %V 34 %N 3 %X A new typical and high efficient rooting and transplanting method of Populus davidiana×P.bolleana was set up from hardening off seedlings, moisturizing and pollution prevention in the study. Firstly, put the tissue culture P.davidiana×P.bolleana seedlings in large test tubes with liquid medium for rooting for 12-15 d. Secondly, refine hydroponic seedlings in the aseptic water for 2 d. Then, transplant the seedlings into soil and cover with plastic cups to moisturizing, which will be removed after 15 d. At last, the 17 d old seedlings with soil were transplanted into the field. Using the above method, the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic, simple, small-scaled and high efficient but also suitable for many other woody plants, so it is easy to generalize. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2014.03.015