%0 Journal Article %A CHEN Li-Jing %A SUN Hong-Mei %A WANG Chun-Xia %A WANG Jin-Xia %A ZHAO Shuang %T Optimization on Extractive Technique of Phenylalanine Ammonia-lyase in Lily Bulb %D 2008 %R 10.7525/j.issn.1673-5102.2008.05.019 %J Bulletin of Botanical Research %P 594-598 %V 28 %N 5 %X Optimum extractive technique of phenylalanine ammonia-lyase(PAL) in the bulb of Lilium davidii var. unicolor was studied. The results indicated that Borax solution with pH8.8 was the best buffer. PAL in lily bulb was not able to endure the strong acid and alkali, especially the former. The PAL activity declined gradually along with the increase of temperature and prolonging of bath duration, but the activity still remained 47% after 30 min in 40℃, and it was the optimum bath temperature. 0.032 mol·L-1 substrate was the best, and grinding with 0.5 g PVP could improve the PAL activity obviously. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2008.05.019