%0 Journal Article %A Qiu-Jin TAN %A Shu-Fang ZHENG %A Tao ZHANG %A Wen-Lin WANG %A Xiu-Hua TANG %A Yuan-Rong WEI %T Cloning and Expression Vector Construction of a Flowering Related Gene MiMADS-box from Macadamia integrifolia %D 2021 %R 10.7525/j.issn.1673-5102.2021.06.006 %J Bulletin of Botanical Research %P 888-895 %V 41 %N 6 %X

In order to clarify the mechanism of MADS-box transcription factor gene family member in flowering and floral organ development in Macadamia integrifolia, aMiMADS-box gene was cloned from the leaves of M. integrifolia “GUIRE1” cultivar by PCR technique and analyzed its structure and function by Bioinformatics method. The cloned cDNA sequence of MiMADS-box gene was 1 179 bp in length, 729 bp open reading frame (ORF), encoded 242 amino acids. MiMADS-box, an unstable hydrophilic protein that had no transmembrane structure, no signal peptide and localized in the nucleus, had MADS superfamily and K-box superfamily conserved domains belonging to the MIKCc-type family. Phylogenetic analysis clustered MiMADS-box and AGL7/AP1, AGL10/CAL1, AGL8/FUL, AGL79 into FUL-AP1(SQUA) subfamily. The transcriptome data were used to analyze the expression patterns of MiMADS-box gene in branch, flower and leave of M. integrifolia “GUIRE1” and “695” cultivar. Results indicated that MiMADS-box had the lowest expression in the flower of “GUIRE1”, but the expression level of “695” was the highest. The green fluorescent protein fusion vector pGREEN-MiMADS-box-ORF-GFP was constructed by homologous recombination. It was speculated that MiMADS-box was closely related to flowering and floral organ development. The study provided a theoretical and technical guide for clarification the role MiMADS-box in flowering regulation mechanism of M. integrifolia.

%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.06.006