%0 Journal Article
%A Fang CHEN
%A Guo-Liang LI
%A Hao-Tian GAO
%A Jian WANG
%A Lin ZHANG
%A Ting-Ting SHE
%T Mechanism of Sm-miR858 Negatively Regulateda R2R3-MYB Transcription Factor SmPAP1 in Salvia miltiorrhiza
%D 2021
%R 10.7525/j.issn.1673-5102.2021.04.007
%J Bulletin of Botanical Research
%P 522-530
%V 41
%N 4
%X
MicroRNAs(miRNAs) are a class of non-coding small RNAs that negatively regulate gene expression. Through high-throughput sequencing miRNAsof Salvia miltiorrhiza, a mature sequence miR858 named Sm-miR858 was obtained. The sequence alignment showed that the Sm-miR858 was highly conserved with the identified miR858 sequences in other plants, and the result of Small RNA northern blotting showed that the Sm-miR858 was expressed in the root, stem and leaf tissues of S. miltiorrhiza, especially relatively high in leaves. To explore the function of Sm-miR858 in S. miltiorrhiza, the Sm-miR858 was predicted first online using biological software. The result of psRNATarget analysis showed that there were 13 potential target genes of Sm-miR858. One was SmPAP1, an important transcription factor, participated in the metabolic regulation on phenolic acid active substances of S. miltiorrhiza. To further verify the targeting effect of Sm-miR858 on SmPAP1, the correlation of co-expression between Sm-miR858 and SmPAP1 was analyzed and verified by Real-time qPCR in the tobacco transient expression system and S. miltiorrhiza tissues and organs. The Real-time qPCR results showed that there was a significant negative correlation co-expression between SmPAP1 and Sm-miR858 in S. miltiorrhiza. Then the Sm-miR858 and SmPAP1 over-expressing plant vectors were constructed, and the transient co-expression of the Sm-miR858 and SmPAP1 was subsequently assayed in tobacco. The results showed that the sm-mir858 overexpression resulted in a significant decrease of the SmPAP1 mRNA expression level, compared with the control, and this indicated that sm-mir858 did actually target and negatively regulate the expression of SmPAP1 in S. miltiorrhiza. Above results laid a solid foundation for elucidating the role of sm-mir858 in the regulation phenolic acids biosynthesis in S. miltiorrhiza.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.04.007