%0 Journal Article %A CHEN Shi-Long %A CHI Xiao-Feng %A GAO Qing-Bo %A LIU Hai-Rui %A XING Rui %A ZHANG Fa-Qi %T Gnentic Diversity and Phylogeographic Structure of Triosteum pinnatifidum based Chloroplast DNA Sequence rbcL-accD %D 2018 %R 10.7525/j.issn.1673-5102.2018.02.016 %J Bulletin of Botanical Research %P 278-283 %V 38 %N 2 %X Triosteum pinnatifidum is perennial herb belonging to Triosteum (Caprifoliaceae) with the distribution in northwest Qinghai-Tibetan Plateau and adjacent mountain regions and Japan. It's endemic species of East Asia. We studied the sequence features, genetic variation and phylogeographic structure of T.pinnatifidum based on chloroplast DNA intergenic region rbcL-accD. Analysis of molecular variance for the whole populations revealed that 72.28% of total genetic variation occurred among populations, and 27.72% within populations. Dominant haplotype H2 had wide distribution while the private haplotypes could be found in different populations. There was no phylogeographic structure(NST<GST) and low gene flow(Nm=0.096), indicating the geographic isolation and variable habitat created by the special topography or ecological environment on the Qinghai-Tibetan Plateau and adjacent mountain regions may form current distribution of haplotypes. T.pinnatifidum experienced nonsignificant species selection or expansion. Indeed, we speculate there were different refugias for T.pinnatifidum during glaciations, it spread to current locations during interglaciation or postglaciation. To know the details of refuges and the migration routes, more work are needed in future survey. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.02.016