%0 Journal Article %A DU Ming-Lin1 %A HU Zhang-Wei1 %A HUANG Zuo-Xi1 %A LI Fang1 %A LIN Zhong-Quan2 %A XIAO Xiao-Jun1* %T Establishment and Optimization of Wild Beltilla striata Regeneration System %D 2015 %R 10.7525/j.issn.1673-5102.2015.06.006 %J Bulletin of Botanical Research %P 825-831 %V 35 %N 6 %X We used the ripe capsules from wild Bletilla striata as explants to establish regeneration system. The optimal medium for seed germination was 1/2MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1. The most suitable medium for PLB multiplication was MS+KT 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1, while MS+6-BA 0.5 mg·L-1+KT 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1 was appropriate for PLB differentiation. The optimal medium for adventitious buds multiplication was MS+6-BA 0.5 mg·L-1+KT 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1+potato juice 150 g·L-1, and the proliferation rate was up to 343% after 30 days. The most suitable rooting medium was 1/2MS+IBA 0.5 mg·L-1+AC 0.5 g·L-1+potato juice 100 g·L-1 with the rooting rate of 100%. The best way of tube seedlings transplanting was under the volume ratio of sphagna: batk: Coconut chaff: wood flour of 2∶2∶1∶1 with 99% of survival rate. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2015.06.006