%0 Journal Article
%A JIANG Jing
%A JIANG Yan-Cheng
%A LIU Ming-Ying
%A QIAO Gui-Rong
%A YANG Hui-Qin
%A ZHUO Ren-Ying*
%T Construction of Expression Vector of ptc-miR801 Gene and Function Analysis
%D 2013
%R 10.7525/j.issn.1673-5102.2013.05.017
%J Bulletin of Botanical Research
%P 599-604
%V 33
%N 5
%X ptc-miR801 was identified from P.cathayana by microRNA library sequence, and it had been confirmed that ptc-miR801 was induced by salt stress. The putative targets were NAC-domain protein and Scarecrow-like gene, which were shown to play important roles in response to salt stress. To investigate the function of ptc-miR801, the artificial miR801 plant expression vector was constructed and transformed into Arabidopsis thaliana. Transformed plants were confirmed by PCR and RT-PCR. The results showed that amiRNA transcript level was markedly improved in transgenic plant compared with non-transgenic plants and the overexpression of ptc-miR801 could enhance the salt tolerance of transgenic plants. Our results would help to clarify the molecular mechanism of P.cathayana under salt stress.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2013.05.017