%0 Journal Article %A HU Chun %A LI Fu-Xiang %A LIU Feng-Mei %A LIU Zuo-Jun* %T Detection Methods for Pollen Viability of Anemone obtusiloba %D 2013 %R 10.7525/j.issn.1673-5102.2013.05.014 %J Bulletin of Botanical Research %P 582-586 %V 33 %N 5 %X The pollen viability of Anemone obtusiloba with three-olor flowers (yellow, pale yellow and white) was detected with four methods: TTC dyeing, I2-I dyeing, inorganic acid test, sucrose-ased germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that: (1) The fertile rate of pollen of A.obtusiloba is high, about 80%. Pollens diameter are 28.75-33.75 m. (2) The concentration of TTC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. (3) I2-I dyeing is not ideal, dyeing rate is only 8.33%-22.2%, and there is no significant difference within three-olor flowers. (4) The pollen vitality of A.obtusiloba with three-olor flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TTC dyeing is a simple and rapid determination method of A.obtusiloba. Within a day, pollen vitality is maximum from 12:00-14:00 pm. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2013.05.014