%0 Journal Article %A LI Chun-Ying %A Liu De-Man %A Liu Shuai-Hua %A ZHANG Bao-You %A ZHAO Chun-Jian %A ZU Yuan-Gang %T Optimization of Homogenate Extraction Technology of CoQ10 in Tobacco Leaves by Orthogonal Experiments %D 2012 %R 10.7525/j.issn.1673-5102.2012.03.022 %J Bulletin of Botanical Research %P 373-376 %V 32 %N 3 %X Using tobacco leaves as materials, the homogenate extraction technology of coenzyme Q10 (CoQ10) in tobacco was optimized by orthogonal experiments. The effect of homogenate time, ethanol volume percent, ratio of liquid to material and number of extraction cycles on the extraction yield of CoQ10 was investigated. The optimized homogenate extraction technology was determined as follows: 85% ethanol as extraction solvent, homogenate time 6 min,ratio of liquid to material 9∶1(mL· g-1), 2 extraction cycles. A comparison between homogenate and Soxhlet extractions was conducted. The extraction yield of CoQ10 was 12.82 μg·g-1 by homogenate extraction, which was almost equivalent to Soxhlet extraction. Homogenate extraction is faster and needs less solvent, thereby homogenate extraction has an advantage over Soxhlet extraction. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2012.03.022