%0 Journal Article %A CHEN Xu-Hui %A GAO Yu-Bao* %A ZHAO Ting-Ting %A ZHU Min-Jie %T Genomic DNA Extraction and AFLP Analysis System Establishment of Caragana microphylla %D 2009 %R 10.7525/j.issn.1673-5102.2009.05.004 %J Bulletin of Botanical Research %P 529-533 %V 29 %N 5 %X The genomic DNA in the fresh leaves of Caragana microphylla was extracted using a modified SDS method, and then an AFLP silver-stained analysis system suitable for C.microphylla was established by optimizing several main factors. We found that the genomic DNA obtained had good purity and integrity for AFLP analysis with mercapto-ethanol in the extracting solution to prevent formation of oxides and PVP to eliminate phenol from genomic DNA. 500 ng genomic DNA was digested for 4 h with EcoRⅠand MseⅠrestriction enzymes, ligated to adaptors for 12 h at 16℃, pre-amplified using EcoRⅠ+1 and MseⅠ+1 primers and then amplified using EcoRⅠ+3 and MseⅠ+3 primers. The amplified products were resolved in denaturing ployacrylamide gels and stained with silver. With this system, clear ploymerphic fingerprints were obtained, which can be taken as a base for future study of AFLP for C.microphylla populations. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2009.05.004