Abstract: The influence of UV wavelength, time of radiation and the processing method of sample on the content of paclitaxel and cephalomannine in the fresh leaves of Taxus cuspidata was studied in the article, in which two wavelength of 254 nm and 365 nm were used. The results showed that two kinds of UV radiation could enhance the content of two compounds in the fresh leaves of Taxus cuspidata by radiating the water homogenate of the fresh leaves. But, the increase trend of two compounds was different under diverse UV radiation. The wavelength of 365 nm made the content of two compounds achieve the maximum in 2 hours. And the rate of increase were 44.6% for paclitaxel and 53.0% for cephalomannine. While the wavelength of 254 nm needed 8 hours. The rate of increase were 39.2% for paclitaxel and 24.3% for cephalomannine. Thus，the content of paclitaxel and cephalomannine in the fresh leaves of Taxus cuspidata can be enhanced rapidly and effectively by radiating the water homogenate of the fresh leaves under UV radiation.

Key words: Taxus cuspidata, paclitaxel, cephalomannine, UV radiation