Effects of Spraying NaHS at Different Growth Stages on H2S Production and Reactive Oxygen Species Metabolism of Naked Oat Leaves under Saline-Alkali Stress

doi:10.7525/j.issn.1673-5102.2022.03.015

Abstract

Abstract:

In order to illuminate the regulating mechanism of gas signal hydrogen sulfide（H₂S） on reactive oxygen species（ROS） metabolism of naked oat（Avena nuda） under saline-alkali stress， and to screen and determine the optimal application period and concentration of H₂S， the effect of spraying NaHS（H₂S donor； 0， 25， 50， 100， 200 and 400 μmol·L^-1） at different periods（seedling stage， jointing stage， heading stage， flowering stage and filling stage） on the H₂S content， H₂S production related enzyme activityof L-cysteine desulfhydrylase（LCD） and ROS metabolism-related enzyme activities and substance contents in leaves of soil-potted naked oat under 3.0 g·kg^-1 salt-alkali mixed stress（NaCl∶Na₂SO₄∶NaHCO₃∶Na₂CO₃ molar ratio was 12∶8∶9∶1） were carried out respectively. The results showed that spraying period， NaHS concentration and their interaction had significant effects on the content of H₂S， superoxide anion（）， hydrogen peroxide（H₂O₂）， malondialdehyde（MDA）， ascorbic acid（AsA） and glutathione（GSH）， and the activities of LCD， superoxide dismutase（SOD）， catalase（CAT）， peroxidase（POD）， ascorbic acid peroxidase（APX） and glutathione reductase（GR） in naked oat leaves under salt-alkali stress respectively. Compared with spraying 0 μmol?L^-1 NaHS， spraying a certain concentration of NaHS increased the contents of H₂S， AsA，GSH and the activities of LCD， SOD， CAT， POD， APX and GR， and reduced the accumulation of ， H₂O₂ and MDA respectively. However， the optimal spraying period and NaHS concentration of the above indexes were different. Comprehensive analysis of membership function showed that spraying 25-200 μmol?L^-1 NaHS at the seedling stage and jointing stage had the highest the comprehensive evaluation value（D）. It indicated that spraying 25-200 μmol?L^-1 NaHS at the seedling-joint stage improved the ROS scavenging ability， alleviated the oxidative damage of salt-alkali stress on naked oats.

Key words: hydrogen sulfide, naked oat, saline-alkali stress, reactive oxygen species metabolism

CLC Number:

S543.603.4

Jianxin LIU, Ruirui LIU, Xiuli LIU, Haiyan JIA, Ting BU, Na LI. Effects of Spraying NaHS at Different Growth Stages on H₂S Production and Reactive Oxygen Species Metabolism of Naked Oat Leaves under Saline-Alkali Stress[J]. Bulletin of Botanical Research, 2022, 42(3): 455-465.

Figures/Tables 14

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NaHS浓度 NaHS concentrations /（µmol∙L^-1）	H₂S含量 H₂S content /（µmol∙g^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	26.95±3.45cC	34.47±3.48bB	31.98±3.65bB	46.35±3.14aA	31.47±3.10bC
25	24.45±1.49dC	36.19±2.51bcB	33.28±2.43cB	39.67±2.70bB	45.56±3.79aB
50	44.58±1.74aA	34.83±4.97bB	42.02±4.99aA	36.68±4.55bBC	42.31±4.51aB
100	38.86±3.81cB	45.61±3.79bA	43.36±3.48bcA	40.04±2.66cB	61.49±4.50aA
200	41.46±3.53aAB	43.54±2.83aA	35.55±2.10bB	44.07±3.41aA	45.67±2.76aB
400	26.33±3.10cC	41.53±3.43aA	41.49±3.07aA	34.63±3.12bC	45.73±2.50aB

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	LCD活性 LCD activity /（μg∙g^-1∙min^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	458.1±58.7cC	585.9±59.1bB	543.7±62.1bB	788.0±53.4aA	555.1±34.9bD
25	415.7±25.3dC	615.3±42.7bcB	565.9±41.3cB	674.4±45.9bBC	813.3±40.2aB
50	757.9±29.6aA	592.2±84.5cB	714.4±84.7abA	663.6±34.3bCD	731.1±58.2abC
100	660.6±64.8cB	775.4±64.4bA	737.1±59.3bcA	700.8±28.0bcBC	1 035.1±58.7aA
200	704.8±60.0aAB	740.2±48.2aA	604.4±35.7bB	749.1±57.9aAB	776.3±46.9aBC
400	447.7±52.7cC	706.0±58.4aA	705.2±52.2aA	588.7±53.0bD	777.4±42.5aBC

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	含量content /（μg∙g^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	4.79±0.57cdB	3.41±0.93dAB	5.94±0.71bcC	6.58±1.23bA	8.67±1.22aBC
25	4.86±0.67cAB	3.07±0.81dAB	6.62±0.42bBC	5.58±0.88bcAB	10.13±1.48aA
50	6.35±0.81aA	2.64±0.51bB	6.31±0.76aBC	4.05±0.69bB	6.95±0.72aD
100	4.42±1.20bB	3.71±0.86bAB	7.82±1.17aB	4.20±0.88bB	7.53±1.44aC
200	4.16±0.74cB	4.21±0.83cA	5.87±2.42bC	4.63±0.67bcB	9.57±0.75aAB
400	5.57±1.03cAB	4.26±0.70cA	11.05±1.34aA	5.10±1.02cAB	9.01±1.85bAB

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	H₂O₂含量 H₂O₂ content /（μmol∙g^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	110.8±21.4bBC	104.5±14.0bA	178.7±29.4aA	81.1±11.3cA	45.2±7.5dA
25	92.2±9.1bC	95.9±19.8bAB	128.7±20.8aCD	69.4±13.4cA	63.9±21.6cA
50	129.6±12.1aAB	78.8±13.9bBC	146.7±13.4aBC	60.0±11.7bcA	53.8±14.2cA
100	116.7±19.3aAB	58.0±4.6bC	126.5±13.9aCD	65.5±10.2bA	45.2±3.3bA
200	119.4±21.3aAB	60.0±8.3bC	122.5±21.9aD	73.3±7.6bA	51.8±10.9bA
400	136.5±14.8bA	69.9±10.3cdC	162.3±13.4aAB	78.1±20.9cA	53.4±16.5dA

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	MDA含量 MDA content /（nmol∙g^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	11.21±3.69dA	17.00±2.69cA	26.78±3.41bA	36.30±3.59aA	36.54±3.01aA
25	9.07±1.74dA	18.41±2.05cA	24.33±3.82bAB	38.32±4.51aA	24.55±4.33bD
50	10.69±3.18cA	16.97±2.19bA	20.90±5.71bB	28.36±7.06aB	33.05±5.07aAB
100	9.81±0.48dA	16.30±3.62cA	28.91±3.31bA	40.90±2.62aA	27.30±2.99bCD
200	8.67±1.54dA	13.47±2.21dA	26.90±5.61cA	42.19±3.80bA	32.89±5.24aABC
400	11.78±1.54eA	17.56±4.45dA	23.10± 4.95cAB	36.90±3.95aA	29.39±8.53bBCD

Effects of Spraying NaHS at Different Growth Stages on H₂S Production and Reactive Oxygen Species Metabolism of Naked Oat Leaves under Saline-Alkali Stress

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因子 Factor	F
因子 Factor	H₂S	LCD		H₂O₂	MDA	SOD	CAT	POD	APX	ASA	GSH	GR
喷施时期Spraying period	45.6^*	60.3^*	109.6^*	174.5^*	211.0^*	69.1^*	252.6^*	403.4^*	21.5^*	176.3^*	90.2^*	10.8^*
NaHS浓度Concentration	39.4^*	44.9^*	8.1^*	7.3^*	2.7^*	20.1^*	15.6^*	9.0^*	4.8^*	2.9^*	6.2^*	7.9^*
交互作用Interaction	15.9^*	17.5^*	6.2^*	4.5^*	3.6^*	5.4^*	14.4^*	5.0^*	7.9^*	11.0^*	7.9^*	6.4^*

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[9]	ZHAO Min, YANG Ning, CHEN Lu, AN Yan-Huang, LI Wen-Ling, ZHAO Feng-Feng. Effects of H₂S and PLDα1 on Drought Stress in Arabidopsis thaliana [J]. Bulletin of Botanical Research, 2018, 38(3): 406-414.
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NaHS浓度 NaHS concentrations /（µmol∙L^-1）	SOD活性 SOD activity /（U∙g^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	395.0±9.0ABa	329.2±23.4Bc	403.9±9.4Aa	364.6±20.4ABb	399.3±5.8Aa
25	393.7±9.5ABa	295.9±32.8Cc	387.5±6.5Aa	339.9±26.5Cb	351.4±5.5Cb
50	364.2±4.9Ca	323.4±16.7Bb	323.7±28.5Bb	352.5±15.9BCb	367.4±5.6BCa
100	392.1±11.3Ba	317.2±28.8Bb	380.2±8.4Aa	383.0±9.9Aa	379.0±11.0ABa
200	400.9±12.8ABa	365.4±9.7Abc	385.6±11.2Aab	360.3±11.9ABCc	367.1±4.6BCbc
400	416.1±6.7Aa	338.4±23.9Bc	393.2±9.9Aab	384.0±18.6Ab	378.0±21.8ABb

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	CAT活性 CAT activity /（U∙g^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	2787.8±398.2Ba	1561.6±378.6Ab	1167.0±128.6Bc	409.6±72.1Ad	387.2±185.4Ad
25	3408.0±409.6Aa	1564.8±414.4Ab	1310.7±274.9Bb	368.0±33.9Ac	326.4±90.9Ac
50	2432.0±202.7Ca	1340.8±412.5Ab	1724.2±339.7Ab	422.4±128.3Ac	499.2±96.3Ac
100	2880.0±512.5Ba	1718.4±601.3Ab	1009.0±134.8BCc	489.6±78.9Ad	355.2±59.2Ad
200	1337.6±363.4Da	1450.6±299.9Aa	713.6±214.2Cb	563.2±130.7Ab	361.6±31.2Ab
400	933.8±141.0Eb	1360.0±257.7Aa	1292.8±136.4Bab	489.6±120.6Ac	540.8±30.8Ac

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	POD活性 POD activity /（U∙g^-1∙min^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	1572.2±198.3Ba	1162.6±264.8Bb	642.6±35.7Ac	60.2±9.4Ad	54.4±10.3Ad
25	1844.5±439.2Aa	1160.6±271.2Bb	657.3±90.5Ac	57.6±9.1Ad	58.6±4.8Ad
50	1573.1±371.4Ba	1205.1±103.9Bb	470.1±82.6Ac	45.8±12.0Ad	55.4±2.4Ad
100	1148.8±325.3Cb	1489.6±158.9Aa	510.4±85.9Ac	42.6±9.2Ad	65.2±10.6Ad
200	1115.8±239.3Ca	1160.0±216.8Ba	510.1±99.9Ab	61.8±11.9Ac	57.6±7.8Ac
400	994.9±217.0Ca	1073.6±181.9Ba	139.0±15.1Ab	56.3±11.6Ab	55.0±6.2Ab

NaHS浓度 NaHS concentrations /（µmol∙L^-1）	APX活性 APX activity /（μmol∙g^-1∙min^-1）
NaHS浓度 NaHS concentrations /（µmol∙L^-1）	幼苗期 Seedling	拔节期 Jointing	抽穗期 Heading	开花期 Flowering	灌浆期 Grouting
0	1.51±0.31Bb	3.67±0.22Aa	2.09±0.60Ab	1.83±0.22Bb	1.95±0.48Cb
25	1.78±0.51ABb	3.72±0.65Aab	2.21±0.47Ab	3.43±0.44Aa	2.21±0.65BCb
50	1.75±0.54ABc	2.08±0.55Bc	2.04±0.25Ac	2.91±0.80Ab	4.06±0.29Aa
100	1.83±0.34ABb	1.97±0.12Bb	1.89±0.41Ab	3.09±0.57Aa	2.79±0.64Ba
200	1.54±0.30Bc	2.26±0.29Bbc	1.85±0.83Abc	3.02±0.56Aa	2.52±0.87BCab
400	2.42±0.55Aab	1.66±0.45Bb	1.86±0.19Ab	2.71±0.71Aa	1.80±0.35Cb