Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene HbRGA1 in Rubber Tree

doi:10.7525/j.issn.1673-5102.2021.04.008

Abstract

Abstract:

DELLA protein is a negative regulator of gibberellin signaling pathway， which inhibits plant growth and development. Analyzing the structure and function of DELLA family members will be helpful to elucidate the mechanism by which the DELLA protein regulates the growth and development of rubber trees. In this study， the full-length cDNA sequence of HbRGA1 was cloned from the leaves of rubber tree cultivar Reyan73397. HbRGA1 is 2 136 bp long， contains 1 839 bp ORF， and encodes 613 amino acids. The HbRGA1 protein sequence contains DELLA and GRAS superfamily conserved domains， which are 82.5% like the DELLA genes of poplar， cassava and rubber trees. qRT-PCR analysis showed that the expression level of HbRGA1 was high in the leaf of rubber tree， but very low in the bark and latex. The expression of HbRGA1 in leaves was significantly up-regulated by gibberellin and abscisic acid spraying. These results indicated that HbRGA1 was closely related to gibberellin and other hormone signals in rubber trees， which laid a good foundation for further study of HbRGA1’s structure and function in the growth and development of rubber trees.

Key words: Hevea brasiliensis, HbRGA1, gibberellin, gene expression

CLC Number:

S794.1

Hua-Feng CHEN, Song-Le FAN, Li-Feng WANG. Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene HbRGA1 in Rubber Tree[J]. Bulletin of Botanical Research, 2021, 41(4): 531-539.

Figures/Tables 7

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引物名称 Primer name	引物序列 Primer sequence(5′—3′)
HbRGA1 F	GAAAGATGAAAAGAGATCACCAAGA
HbRGA1 R	ACTGACTCGCTAACATTAACTCACC
HbRGA1 QF	CCTCTCTTAGCTGATGCTT
HbRGA1 QR	GCGAGTCAATTAAGACAACC
HbACTIN F (Genbank：HQ260674.1)	GATGTGGATATCAGGAAGGA
HbACTIN R	CATACTGCTTGGAGCAAGA

项目 Project	理化性质 Physicochemical property
分子式Formula	C₂₉₁₈H₄₅₉₁N₈₀₃O₉₁₄S₂₉
相对分子质量Molecular weight (kDa)	66476.12
等电点Theoretical pI	5.19
正电荷残基数 Number of positive charge residues (Arg+Lys)	49
负电荷残基数 Number of negatively charge residues(Asp+Glu)	71
不稳定系数Instability index	50.01（大于40为不稳定）
脂肪系数Aliphatic index	82.94
总平均亲水性 Grand average of hydropathicity (GRAVY)	-0.220

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