Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb. Using Response Surface Methodology

doi:10.7525/j.issn.1673-5102.2020.05.017

Abstract

Abstract: Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb. The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction, ultrasound irradiation power, extraction temperature, liquid-solid ratio and ultrasound irradiation time. The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power, ultrasound irradiation time and liquid-solid ratio, and optimized extraction process. The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%, ultrasonic irradiation power of 140 W, ultrasonic irradiation time of 30 min, liquid-solid ratio of 20.5 mL·g^-1, and extraction temperature of 40℃. Under these optimal conditions, the yield of dihydromyricetin was 2.14±0.09 mg·g^-1. The extraction method is simple, rapid, and high in efficiency, which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.

Key words: Hovenia dulcis Thunb., seed, ultrasound-assisted extraction, dihydromyricetin, response surface methodology

CLC Number:

TQ423

ZHANG Xiao-Nan, ZHU Hong-Wei, ZHAO Shan-Bo, BAI Chen, SHI Ya-Nan, XU Hao-Peng, YIN Lian, HU Shu-Min, YIN Hua. Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb. Using Response Surface Methodology[J]. Bulletin of Botanical Research, 2020, 40(5): 775-781.

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