Preparation of Resveratrol-Nanostructured Lipid Carrier and Evaluation of in vitro Antioxidant Activity

doi:10.7525/j.issn.1673-5102.2020.04.017

Abstract

Abstract: Resveratrol is a natural functional component, with whitening, antioxidant and other effects, can be used to improve skin lesions and cell aging. Resveratrol has low solubility and poor stability in water, which impedes its application in medicine and cosmetics. The nanostructured lipid carrier loaded with resveratrol was prepared to improve the stability and functional activity of resveratrol. The resveratrol nanostructured lipid carrier(NLC) was prepared by thermal high pressure homogenization method, and the homogenization pressure was determined to be 600 bar with GMS as the solid lipid, oleic acid as the liquid lipid, with their ratio of 1:1, 4% Tween 80 as the emulsifier, and the smallest particle size of resveratrol lipid carrier was 179 nm after three cycles.The results of MTT experiment and ROS scavenging experiment also showed that resveratrol did not produce obvious toxicity to cells when the lipid carrier concentration of resveratrol was less than 100 μmol·L^-1, and had better antioxidant activity than resveratrol API.

Key words: resveratrol, nanostructure lipid carrier, hot high-pressure homogenization(HPH), particle size, antioxidant

CLC Number:

Q949

WANG Xin-Yu, LI Hong-Kun, YU Liang, ZHAO Xue-Lian, YU Xue-Ying, FU Yu-Jie. Preparation of Resveratrol-Nanostructured Lipid Carrier and Evaluation of in vitro Antioxidant Activity[J]. Bulletin of Botanical Research, 2020, 40(4): 623-628.

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