Component Analysis of Volatile Organic Compounds Released by Branch and Leaf of Quercus variabilis and Response to Mechanical Injury in Beijing Pinggu

doi:10.7525/j.issn.1673-5102.2019.01.005

Abstract

Abstract: The experiment was conducted to study the component of VOCs released by branch and leaf of Quercus variabilis and response to mechanical injury in Beijing Pinggu. The plant volatile organic compounds(BVOCs) from branches and leaves of Q.variabilis were collected and analyzed with GC-MS. Eight kinds of volatile components, such as esters, alkanes, terpenes, aromatic hydrocarbons, aldehydes and ketones, alcohols, alkenes, as well as other N-containing, S-containing and Cl-containing compound were detected. Esters and alkanes were the main components of Q.variabilis, and account for 30.32%-41.60% and 30.02%-33.14% of the total plant volatile organic compounds content. The comparison of undamaged and mechanically-damaged leaves of Q.variabilis indicated that they had similar VOCs, while the components and contents of volatile substances changed. Dibutyl phthalate, 1-Hexanol, 2-ethyl-, Tetradecane, 2,6,10-trimethyl-, Cyclohexane, 2-ethenyl-1, 1-dimethyl-3-methylene-, Tetradecane, Nonadecane, 9-Octadecenamide,(Z)-, Nonanal, Decanal, etc. were the main components. Mechanical damage brings different degree effects on components and relative content of volatile organic compounds of Q.variabilis branches and leaves. Naphthalene, d-Limonene, 1,3,6-Octatriene, 3,7-dimethyl-,(Z)-,4-Hexen-1-ol, acetate, Dibutyl phthalate, Acetic acid, hexyl ester, Benzoic acid, butyl ester, Cyclohexane, 2-ethenyl-1,1-dimethyl-3-methylene-could be induced and their content increased obviously after mechanical damage. Esters and alkanes were the main VOCs components of Q.variabilis. The contents of naphthalene, d-Limonene, 4-Hexen-1-ol, acetate, Dibutyl phthalate were increased obviously after mechanical damage and these substances had certain ecological function and effect on the anti-herbivory. This may be a chemical defense strategy of Q.variabilis in response to herbivory or against external stress. In addition, many components also changed at different degree or small amplitude after mechanical damage. It's to be further studied on their change mechanism and ecological function.

Key words: BVOCs, mechanical injury, defence, secondary forest

CLC Number:

S731

ZHANG Yu-Ting, WANG Xiao-Fei, NI Yan-Yan, LIU Jian-Feng, JIANG Ze-Ping. Component Analysis of Volatile Organic Compounds Released by Branch and Leaf of Quercus variabilis and Response to Mechanical Injury in Beijing Pinggu[J]. Bulletin of Botanical Research, 2019, 39(1): 35-44.

References

1. 杨小琴. 植物挥发性有机物(VOCs)释放及其环境净化效应概述[J]. 湖南城市学院学报:自然科学版,2006,15(4):57-60. Yang X Q. An review on volatile organic compounds semitted from vegetation and their contribution to the environment[J]. Journal of Hunan City University:Natural Science,2006,15(4):57-60.
2. De Moraes C M,Lewis W J,Paré P W,et al. Herbivore-infested plants selectively attract parasitoids[J]. Nature,1998,393(6685):570-573.
3. Hamilton-Kemp T R,Mccracken Jr,Loughrin J H,et al. Effects of some natural volatile compounds on the pathogenic fungi Alternaria alternata and Botrytis cinerea[J]. Journal of Chemical Ecology,1992,18(7):1083-1091.
4. Obara N,Hasegawa M,Kodama O. Induced volatiles in elicitor-treated and rice blast fungus-inoculated rice leaves[J]. Bioscience,Biotechnology,and Biochemistry,2002,66(12):2549-2559.
5. Farmer E E. Surface-to-air signals[J]. Nature,2001,411(6839):854-856.
6. Pare P W,Tumlinson J H. De novo biosynthesis of volatiles induced by insect herbivory in cotton plants[J]. Plant Physiology,1997,114(4):1161-1167.
7. Paré P W,Tumlinson J H. Plant volatiles as a defense against insect herbivores[J]. Plant Physiology,1999,121(2):325-332.
8. Krause S C,Raffa K F. Comparison of insect,fungal,and mechanically induced defoliation of larch:effects on plant productivity and subsequent host susceptibility[J]. Oecologia,1992,90(3):411-416.
9. Loreto F,Förster A,Dürr M,et al. On the monoterpene emission under heat stress and on the increased thermotolerance of leaves of Quercus ilex L. fumigated with selected monoterpenes[J]. Plant,Cell & Environment,1998,21(1):101-107.
10. 崔骁勇,赵广东,刘世荣. 植物源异戊二烯及其生态意义[J]. 应用生态学报,2002,13(4):505-509. Cui X Y,Zhao G D,Liu S R. Phytogenic isoprene and its ecological significance[J]. Chinese Journal of Applied Ecology,2002,13(4):505-509.
11. Lam E,Fukuda H,Greenberg J. Programmed Cell Death in Higher Plants[M]. Kluwer Academic Publishers,2000:101-114.
12. Llusià J,Peñuelas J,Gimeno B S. Seasonal and species-specific response of VOC emissions by Mediterranean woody plant to elevated ozone concentrations[J]. Atmospheric Environment,2002,36(24):3931-3938.
13. Singsaas E L,Sharkey T D. The regulation of isoprene emission responses to rapid leaf temperature fluctuations[J]. Plant,Cell & Environment,1998,21(11):1181-1188.
14. Rennenberg H,Schnitzler J P. Vocs-helping trees withstand global change?[J]. New Phytologist,2002,155(2):197-199.
15. Isebrands J G,Guenther A B,Harley P,et al. Volatile organic compound emission rates from mixed deciduous and coniferous forests in northern Wisconsin,USA[J]. Atmospheric Environment,1999,33(16):2527-2536.
16. Logan B A,Monson R K,Potosnak M J. Biochemistry and physiology of foliar isoprene production[J]. Trends in Plant Science,2000,5(11):477-481.
17. Guenther A,Zimmerman P,Klinger L,et al. Estimates of regional natural volatile organic compound fluxes from enclosure and ambient measurements[J]. Journal of Geophysical Research:Atmospheres,1996,101(D1):1345-1359.
18. Guenther A,Zimmerman P,Wildermuth M. Natural volatile organic compound emission rate estimates for U. S. woodland landscapes[J]. Atmospheric Environment,1994,28(6):1197-1210.
19. Otter L B,Guenther A,Greenberg J. Seasonal and spatial variations in biogenic hydrocarbon emissions from southern African savannas and woodlands[J]. Atmospheric Environment,2002,36(26):4265-4275.
20. Maja M M,Kasurinen A,Holopainen T,et al. Volatile organic compounds emitted from silver birch of different provenances across a latitudinal gradient in Finland[J]. Tree Physiology,2015,35(9):975-986.
21. Vuorinen T,Nerg A M,Syrjälä L,et al. Epirrita autumnata induced VOC emission of silver birch differ from emission induced by leaf fungal pathogen[J]. Arthropod-Plant Interactions,2007,1(3):159-165.
22. Blande J D,Korjus M,Holopainen J K. Foliar methyl salicylate emissions indicate prolonged aphid infestation on silver birch and black alder[J]. Tree Physiology,2010,30(3):404-416.
23. Maja M M,Kasurinen A,Yli-Pirilä P,et al. Contrasting responses of silver birch VOC emissions to short-and long-term herbivory[J]. Tree Physiology,2014,34(3):241-252.
24. Wenke K,Kai M,Piechulla B. Belowground volatiles facilitate interactions between plant roots and soil organisms[J]. Planta,2010,231(3):499-506.
25. 平立岩,沈应柏. 植物创伤诱导的挥发物及其信号功能[J]. 植物生理学通讯,2001,37(2):166-172. Ping L Y,Shen Y B. Plant wound-induced volatiles and their signal functions[J]. Plant Physiology Communications,2001,37(2):166-172.
26. 郑浩,杨长举,华红霞,等. 与昆虫有关的植物挥发性次生物质的研究方法[J]. 昆虫知识,2002,39(1):9-13. Zheng H,Yang C J,Hua H X,et al. Research methods on plant volatile semiochemicals in insect-plant interactions[J]. Entomological Knowledge,2002,39(1):9-13.
27. 孔垂华,徐涛,胡飞,等. 环境胁迫下植物的化感作用及其诱导机制[J]. 生态学报,2000,20(5):849-854. Kong C H,Xu T,Hu F,et al. Allelopathy under environmental stress and its induced mechanism[J]. Acta Ecologica Sinica,2000,20(5):849-854.
28. 史胜青,雷静品,胡永建,等. 树木挥发物及其抗虫作用研究进展[J]. 华北农学报,2010,25(S1):295-300. Shi S Q,Lei J P,Hu Y J,et al. Perspective of VOCs emission and its functions on defence of insects in trees[J]. Acta Agriculturae Boreali-Sinica,2010,25(S1):295-300.
29. 黄爱葵,李楠. 植物源挥发性有机物的生态意义(综述)[J]. 亚热带植物科学,2011,40(3):81-86. Huang A K,Li N. The ecological significance of botanical volatile organic compounds[J]. Subtropical Plant Science,2011,40(3):81-86.
30. 孙明,刘华,张启翔,等. 3个地被菊品种香气成分的分析[J]. 沈阳农业大学学报,2008,39(1):92-95. Sun M,Liu H,Zhang Q X,et al. Preliminary studies on major aroma ingredients of three ground-cover chrysanthemum cultivars[J]. Journal of Shenyang Agricultural University,2008,39(1):92-95.
31. 黄儒珠,檀东飞,张建清,等. 3种南洋杉科植物叶挥发油的化学成分[J]. 林业科学,2008,44(12):99-104. Huang R Z,Tan D F,Zhang J Q,et al. Chemical constituents of leaf volatile oil from three Araucariaceae[J]. Scientia Silvae Sinicae,2008,44(12):99-104.
32. 任露洁,王成,古琳,等. 无锡惠山森林公园香樟林内挥发物成分及其变化研究[J]. 中国城市林业,2012,10(3):8-11. Ren L J,Wang C,Gu L,et al. VOCs components in Cinnamomum camphora forest and their variation in Hui Mountain Forest Park,Wuxi[J]. Journal of Chinese Urban Forestry,2012,10(3):8-11.
33. 董建华,王成,张晶,等. 女贞枝叶机械损伤前后挥发性物质的变化[J]. 中国城市林业,2011,9(3):40-43. Dong J H,Wang C,Zhang J,et al. Changes of VOCs released by branch and leaf of Ligustrum lucidum Ait. after mechanical damage[J]. Journal of Chinese Urban Forestry,2011,9(3):40-43.
34. 高群英. 9种常见绿化植物VOCs及光合特性研究[D]. 杭州:浙江农林大学,2011. Gao Q Y. The research of 9 common plants VOCs and photosynthetic characteristics[D]. Hangzhou:Zhejiang A&F University,2011.
35. 何培青,柳春燕,郝林华,等. 植物挥发性物质与植物抗病防御反应[J]. 植物生理学通讯,2005,41(1):105-110. He P Q,Liu C Y,Hao L H,et al. Volatile organic compounds and plant defence against pathogenic disease[J]. Plant Physiology Communications,2005,41(1):105-110.
36. 严善春,张丹丹,迟德富. 植物挥发性物质对昆虫作用的研究进展[J]. 应用生态学报,2003,14(2):310-313. Yan S C,Zhang D D,Chi D F. Advances of studies on the effects of plant volatiles on insect behavior[J]. Chinese Journal of Applied Ecology,2003,14(2):310-313.
37. 何翠娟,张颂函. d-柠檬烯生物增效杀虫剂在农业生产上的应用和开发前景[J]. 世界农药,2008,30(4):30-32,50. He C J,Zhang S H. The application and development prospect of d-limonene biosynergistic insecticide in agricultural production[J]. World Pesticides,2008,30(4):30-32,50.
38. 王文慧,杨怀林,闻鸣,等. 3种化学物质对昆虫的趋避与吸引作用研究[J]. 安徽农学通报,2015,21(3-4):29-32. Wang W H,Yang H L,Wen M,et al. Study on three chemicals for insect avoidance and attraction[J]. Anhui Agricultural Science Bulletin,2015,21(3-4):29-32.
39. 刘小秧,程桂荪. 酞酸二丁酯对作物及微生物的影响[J]. 中国环境科学,1992,12(2):158-160. Liu X Y,Cheng G S. Effect of dibutyl pathalate on crops and microorganisms[J]. China Environmental Science,1992,12(2):158-160.
40. 张前龙. 酞酸二丁酯的检测及其对果蝇的毒性研究[D]. 上海:同济大学,2005. Zhang Q L. Determination of di-n-butyl phthalate and the toxicological study on Drosophila melanogaster[D]. Shanghai:Tongji University,2005.

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