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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (4): 397-402.doi: 10.7525/j.issn.1673-5102.2011.04.004

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Optimization of Method for Extraction and Isolation of β-conglycinin from Soybean Seed

SI Xiao-Xia;WANG Zhi;HUO Fei;SHI Tong-Lin;FU Yue-Jun;TIAN Huai-Dong*   

  1. 1.College of Life Science,Shanxi University,Taiyuan 030006;2.Shanxi Institute of Crop Germplasm Resources,Taiyuan 030031;3.Institute of Biotechnology,Shanxi University,Taiyuan 030006
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
  • Contact: TIAN Huai-Dong
  • Supported by:

Abstract: β-conglycinin, one of the major storage proteins in soybean seeds, greatly influences the quality of soybean seed proteins. However, increasing the purity of the protein has always been one main problem affecting its characterization and identification. In this study, the purity of β-conglycinin was obviously increased by performing condition optimization of the two key links in the process of extraction and isolation of the protein, based on the method reported by Liu et al. Firstly, SBS at increased concentrations was added to the protein solution before the removing of glycinin (11S), the effect of concentration increase on the purity of β-conglycinin was analyzed, and the optimal concentration was determined as 0.03 mmol·L-1; Secondly, the mixed glycinin was removed once more by the water precipitation/centrifugation method before precipitating β-conglycinin. The purity of β-conglycinin finally increased to 98% after optimization. The results have important significance for the characterization and identification of β-conglycinin in the genetic research and breeding for soybean proteins.

Key words: soybean, &beta, -conglycinin, extraction and isolation, optimization

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