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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (6): 941-946.doi: 10.7525/j.issn.1673-5102.2017.06.018

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The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

YANG Ming-Fei1, ZHANG Jing3, SU Wen2, WANG Peng3, ZHANG Sun-Dong3, KOU Ping3, JIAO Jiao3, YANG Yu-Chun4, FU Yu-Jie3   

  1. 1. Research and Development Department of Kitanihon Pharmaceutical CO., No. 55, Kamiichi, Nakaniikawa District, Toyama, Japan 930-0314;
    2. Management Center of Public Medical and Health Institutions in Doumen District, Zhuhai City, Guangdong Province, Zhuhai 519125;
    3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    4. Forestry Academy of Jilin Province, Changchun 130033
  • Received:2017-03-15 Online:2017-11-15 Published:2017-11-25
  • Supported by:
    "13th Five-Year" The National Key Research Projects(2016YFD0600805)

Abstract: As the single active ingredient is poor and prone to drug resistance on effects of lose weight and lipid-lowering, so the study focuses on screening and optimizing the optimal concentration ratio of pinoleic acid/L-carnitine to reduce the lipid of HepG2 cell, which provides the basic data for the development of pinoleic acid lipid-lowering products. Oleic acid induced HepG2 cell adipogenesis, which was used to simulate the model of nonalcoholic fatty liver in vitro. The optimal concentration in combination of pinoleic acid/L-Carnitine decrease lipid was determinated by oil red O staining. The results show that the optimum concentration of oleic acid-induced HepG2 cell steatosis is 0.5 mmol·L-1.The optimal lipolysis concentration of pinoleic acid is 6.25 μmol·L-1. The optimal lipid metabolism concentration of L-Carnitine is 250 μmol·L-1. The best lipid-lowering ratio of pinoleic acid/L-carnitine(PLA/LC) is 1:40(μmol·L-1/μmol·L-1). The characteristics of pinoleic acid and L-Carnitine complexes decreased the doses and improved synergistic effect, Therefore, Combination of pinoleic acid/L-Carnitine has a potential prospectin in the development of lipid-lowering diet products.

Key words: pinoleic acid, L-Carnitine, complexes, lipid-lowering, nonalcoholic fatty liver disease

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