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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (3): 395-401.doi: 10.7525/j.issn.1673-5102.2017.03.010

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Cloning and Expression Analysis of ThPP2C Gene from Tamarix hispida

WANG Pei-Long, LIU Zhong-Yuan, ZHANG Teng-Qian, TANG Fei-Fei, QU Guan-Zheng, GAO Cai-Qiu   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2017-01-03 Online:2017-05-15 Published:2017-06-03
  • Supported by:
    Science and technology research project of Heilongjiang Provincial Department of Education(12523017)

Abstract: Protein phosphatase 2C(PP2C) is a kind of serine/threonine protein phosphatase, which is present in the body as monomer. The reversible phosphorylation by PP2C catalytic is an important component of the cell signal transduction, which is involved almost all the physiological and pathological processes. A novel PP2C gene(ThPP2C) was isolated from Tamarix hispida by RT-PCR. The opening reading frame(ORF)length of ThPP2C was 869 bp, encoding 282 amino acid. The relative molecular weight of ThPP2C protein was 30.54 kD, with isoelectric point 7.13. By the real-time RT-PCR, the expressions of ThPP2C were obviously difference in roots and leaves of T.hispida under the treatments(0.4 mol·L-1 NaCl, 20%(w/v) PEG6000, 150 μmol·L-1 ABA or 100 μmol·L-1 JA) compared to the control. The expression patterns of ThPP2C were not exactly similarity among these treatments. Particularly, the expression of ThPP2C in leaves of T.hispida after the treatments of NaCl, ABA and JA all were down-regulated. However, it was mainly up-regulated in roots under the treatments of NaCl and JA. With the treatment of ABA, the ThPP2C expression was down-regulated at the early stage and then gradually increased. Different with other three stress, under the treatment of PEG6000, the expressions of ThPP2C were obviously down-regulated in roots. It was not obviously different at the early stress stage in leaves until 48 h, when it was highly induced. Therefore, ThPP2C may play an important role in T.hispida to response the high salt, drought stress and hormones.

Key words: PP2C, Tamarix hispida, salt stress, ABA treatment

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