Cloning and Expression Analysis of Citrate Synthase(CS) Gene in Camellia oleifera

doi:10.7525/j.issn.1673-5102.2016.04.011

Abstract

Abstract: A citrate synthase(CS) gene were isolated from Camellia oleifera by RT-PCR. The full-length cDNA of the CS is 1416 bp in size, encodeding a deduced polypetide of 471 amino acids with estimated molecular weight of 52.74 kD and theoretical isoelectric point of 6.95. Homologous alignment showed that the deduced protein had high identities with the CS proteins of other plants, therefore the gene was named as CoCS(Genbank No.KU161147). By phylogenetic tree analysis, CoCS had close genetic relationships with Rhododendron micranthum and Vitis vinifera. By qRT-PCR, the expression of CoCS in root was induced by phosphate deficiency and increased at first and decreased subsequently. The expression patterns of CoCS were different among different tissues and different cultivars.

Key words: Camellia oleifera, citrate synthase, phosphate deficient, gene cloning, expression analysis

CLC Number:

S794.4

YE Si-Cheng, YAO Xiao-Hua, WANG Kai-Liang, LIN Ping, GONG Hong-En, ZHUO Ren-Ying. Cloning and Expression Analysis of Citrate Synthase(CS) Gene in Camellia oleifera[J]. Bulletin of Botanical Research, 2016, 36(4): 556-564.

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