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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (2): 184-189.doi: 10.7525/j.issn.1673-5102.2016.02.005

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Main Anthocyanin Profiles in Petals of Freesia hybrida

XU Yi-Qian1;YUAN Yuan1;TAO Xiu-Hua2;YANG Juan3;SHI Yi-Min1;TANG Dong-Qin1*   

  1. 1.School of Agriculture & Biology,Shanghai Jiaotong University,Shanghai 200240;
    2.Vegetable and Flower Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200;
    3.Shanghai Floriculture Experimental Farm,Shanghai 201625
  • Online:2016-03-15 Published:2016-05-20

Abstract: We analyzed the anthocyanin profiles in 6 cultivars of freesia(Freesia hybrida). The petal color was observed according to the Royal Horticultural Society Color Card(RHSCC). Petal pigment type was identified by characteristic color reaction. UPLC-PAD and UPLC-Q-TOF-MS were used for qualitative and quantitative analysis of anthocyanins. The flavonoids in petals of all cultivars were detected, while carotenoids were not detected; meanwhile, every cultivar, except for ‘Shangnong Ruxiang’, contained anthocyanins in petals. Among five anthocyanincontained cultivars, the highest anthocyanin content was observed in ‘Shangnong Purple Rose’, followed by ‘Shangnong Chenghong’. Totally 6 anthocyanin components were detected in petals of these cultivars, including Delphinidin 3,5-O-glucoside, Petunidin 3,5-O-glucoside, Delphinidin 3-O-glucoside, Malvidin 3,5-O-glucoside, Petunidin 3-O-glucoside and Malvidin 3-O-glucoside. Three anthocyanin components were detected in petals of ‘Shangnong Zixueqing’ and ‘Shangnong Gongfen’, of which were majorly malvidin and petunidin derivated anthocyanins. Malvidin-anthocyaninswith single or double glucosides was present in petals of ‘Shangnong Zixueqing’, while all anthocyanins in petals of ‘Shangnong Gongfen’ bonded with double glucosides, such as Delphinidin 3,5-O-glucoside, Petunidin 3,5-O-glucoside and Malvidin 3,5-O-glucoside, major Petunidin 3-O-glucoside and minor Delphinidin 3-O-glucoside in petals of ‘Shangnong Purple Rose’. While only one anthocyanin component was detected in petals of ‘Shangnong Golden Queen’ and ‘Shangnong Chenghong’, which was Delphinidin 3,5-O-glucoside and Malvidin 3,5-O-glucoside, respectively.

Key words: freesia, flower color, anthocyanin, UPLC-MS