Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities

doi:10.7525/j.issn.1673-5102.2015.04.025

Abstract

Abstract: We used ultrasonic-assisted extraction(UAE) to extract the total flavonoids from Crataegus pinnatifida leaves, and used Box-Behnken design(BBD) combined with response surface methodology(RSM) to optimize extraction conditions. We determined the effects of four variables in terms of ratio of liquid/solid, temperature, ethanol concentration and time on the total flavonoids contents(TFC). The optimized extraction parameters obtained the highest total flavonoids yield were under the ratio of liquid/solid of 15, 40℃, and 40% of ethanol for 32 min. Under these conditions, the yield was 15.50 mg·g^-1. By in vitro antioxidant assays, the extract exhibited a strong DPPH radical-scavenging in ability with IC₅₀ value of 0.69 mg·mL^-1. Compared with conventional maceration extraction(ME) and heat-refluxing extraction(HE) methods, UAE method showed remarkable advantages in terms of better antioxidant activities. Overall, the present results indicated that UAE was a suitable method for the extraction of total flavonoids from C.pinnatifida leaves and its extracts were potential antioxidant ingredients.

Key words: Crataegus pinnatifida leaves, ultrasonic-assisted extraction, total flavonoids, Box-Behnken design, antioxidant activity

CLC Number:

LUO Meng1,2；SONG Zhuo-Yue1,2；HU Jiao-Yang1,2；MU Fan-Song1,2*；QIAO Qi1,2；ZU Yuan-Gang1,2. Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities[J]. Bulletin of Botanical Research, 2015, 35(4): 632-637.

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