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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (5): 582-586.doi: 10.7525/j.issn.1673-5102.2013.05.014

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Detection Methods for Pollen Viability of Anemone obtusiloba

HU Chun;LIU Zuo-Jun*;LI Fu-Xiang;LIU Feng-Mei   

  1. School of Life Science and Engineering of Lanzhou University of Technology,Lanzhou 730050
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-20 Published:2013-09-20
  • Contact: LIU Zuo-Jun
  • Supported by:

Abstract: The pollen viability of Anemone obtusiloba with three-olor flowers (yellow, pale yellow and white) was detected with four methods: TTC dyeing, I2-I dyeing, inorganic acid test, sucrose-ased germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that: (1) The fertile rate of pollen of A.obtusiloba is high, about 80%. Pollens diameter are 28.75-33.75 m. (2) The concentration of TTC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. (3) I2-I dyeing is not ideal, dyeing rate is only 8.33%-22.2%, and there is no significant difference within three-olor flowers. (4) The pollen vitality of A.obtusiloba with three-olor flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TTC dyeing is a simple and rapid determination method of A.obtusiloba. Within a day, pollen vitality is maximum from 12:00-14:00 pm.

Key words: Anemone obtusiloba, detection methods, fertility, pollen, viability

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