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›› 2006, Vol. 26 ›› Issue (4): 405-410.doi: 10.7525/j.issn.1673-5102.2006.04.017

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Comparative expression analysis of Crlea gene in Catharanthus roseus under drought stress by real-time quantitative PCR

ZU Yuan-Gang;NIE Ming-Zhu;FANG Si-Liang;YU Jing-Hua;GUO Xiao-Rui   

  1. (Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: ZU Yuan-Gang

Abstract: A full-length Crlea(Crlea for Catharanthus roseus late embryogenesis abundant) gene was first isolated from Catharanthus roseus. Gene expression profiles of Crlea gene in leaves and roots under drought stress were monitored by real-time quantitative PCR. The results showed that a similar accumulation pattern of Crlea gene in leaves and roots over the observation period of 0.5 to 8 hours. The expression of Crlea mRNA was strengthened with the prolongation of stress time. In leaves, expression amounts of Crlea gene were 9.984 and 20.431 times higher than that of control respectively at 6 and 8 h. Similarly, the expression amounts of Crlea gene in root obviously increased (2.831 times higher than that of control) at 8 h. Primary results show the expression of Crlea gene is non-tissue-specific and up-regulated under drought stress.

Key words: Catharanthus roseus, drought stress, lea gene, real time quantitative PCR, comparative expression