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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (5): 603-606.doi: 10.7525/j.issn.1673-5102.2009.05.017

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Populus nigra×Populus deltoids “108” Transformed the Chimeric Genes of Spider Insecticidal and Bt

YU Wen-Jing;ZOU Chuan-Shan;WANG Zhi-Ying*;ZHANG Guo-Cai   

  1. (College of Forestry,Northeast Forestry University,Harbin150040)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: WANG Zhi-Ying
  • Supported by:

Abstract: Using the agrobacterium-mediated approach, the anti-insect gene (the chimeric genes of spider insecticidal and Bt) was successfully transformed into the acceptor of Populus nigra×Populus deltoids “108” as explant. Through PCR analysis, the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes; Through PCRSouthern blots analysis, the result showed that the positive rate was 50%, and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids “108” plants successfully.

Key words: Populus nigra×, Populus deltoids &ldquo, 108&rdquo, the chimeric genes of spider insecticidal and Bt, PCR-Southern blots analysis

CLC Number: