Identification of Glutathione S-transferase Gene Family in Sugar Beet and the Response to Cadmium Stress

doi:10.7525/j.issn.1673-5102.2022.05.010

Abstract

Abstract:

To clarify the potential function of Glutathione-S-transferase gene（GST） family in sugar beet（Beta vulgaris） growth and development and abiotic stress， the sugar beet BvGSTs was identified， and the physical and chemical properties， evolutionary relationship， homeopathic elements， chromosome location and transcriptional expression characteristics under cadmium stress were comprehensively analyzed， respectively. The results showed that there were 52 members of BvGSTs in sugar beet genome， which were distributed in seven subfamilies； The molecular weight of the members ranged from 17-28 kDa， and the theoretical isoelectric point（pI） was between 5.16-6.77， and most of the members were located in cytoplasm. There were nine motifs found in BvGSTs， among which motif eight was unique to Phi subfamily. Among all the members， 30 BvGSTs were distributed in eight chromosomes respectively， with four tandem repeats. According to cis-acting element analysis， BvGSTs might be involved in a variety of biotic and abiotic stress responses. Transcriptome analysis showed that all of 52 BvGSTs were related to response of sugar beet to cadmium stress. The expression of most members of Tau subfamily under ground parts was positively regulated by cadmium stress； Phi subfamily above ground parts was significantly induced by cadmium stress， and the expression of Tau family was inhibited. The result of qRT-PCR showed that the transcripts of the four differentially expressed BvGSTs were regulated by cadmium stress， which was consistent with the results of transcriptome sequencing. These results would play a foundamental role in further study on the biological function of sugar beet glutathione S-transferase under cadmium stress.

Key words: sugar beet, genome-wide identification, glutathione S-transferase（GST）, cadmium stress

CLC Number:

S566.3

Xin’ai ZHONG, Shiqi MENG, Wanting Zhou, Qi YAO, Qiong ZHANG, Wang XING, Dali LIU. Identification of Glutathione S-transferase Gene Family in Sugar Beet and the Response to Cadmium Stress[J]. Bulletin of Botanical Research, 2022, 42(5): 790-801.

Figures/Tables 9

Table 1

Table 2

The basic information of BvGSTs gene family in Beta vulgaris L.

亚家族 Subfamily	基因 Gene	亚细胞定位Subcellular localization	氨基酸数 Amino acid number	相对分子质量 Relative molecular mas /Da	等电点 Isoelectric point	不稳定系数 Instability index	外显子数目 Exon number
TCHQD	BVRB_1g010190	Cy	267	31 599.37	8.92	46.44	2
Theta	BVRB_5g100460	Cy	247	28 065.58	9.65	37.41	9
Theta	BVRB_5g100450	Cy	239	27 802.81	5.73	54.99	7
Phi	BVRB_1g012630	Cy	418	47 518.83	5.79	41.10	6
	BVRB_1g014300	Cy	416	47 318.52	5.59	39.94	6
	BVRB_014810	Cy	71	7 857.15	6.53	54.98	3
	BVRB_014820	Cy	98	11 264.67	5.16	51.88	1
	BVRB_7g164100	Cy	216	24 428.89	5.89	32.85	3
	BVRB_006420	Cy	214	24 179.06	6.51	33.96	3
	BVRB_006410	Cy	225	25 273.15	6.14	31.86	3
	BVRB_8g201810	Cy	214	23 874.11	5.51	36.62	7
	BVRB_008080	Cy	214	23 940.40	5.84	30.47	3
	BVRB_7g161860	Cy	213	23 795.32	5.91	30.91	3
	BVRB_9g206010	Cy	213	23 791.30	5.99	32.04	3
Zeta	BVRB_1g019170	Cy	232	26 407.11	5.22	45.21	9
Zeta	BVRB_1g019190	Cy	232	26 498.23	5.22	44.87	2
Tau	BVRB_015440	Cy	222	26 098.20	5.26	40.86	2
	BVRB_4g095790	Cy	223	25 859.81	5.18	42.37	2
	BVRB_4g095830	Cy	215	25 467.44	6.25	36.94	2
	BVRB_4g095800	Cy	218	25 749.73	5.91	35.13	2
	BVRB_6g151700	Cy	220	25 242.17	5.35	35.24	2
	BVRB_4g094330	Cy	225	26 220.12	5.25	49.85	2
	BVRB_4g096480	Cy	131	15 403.84	5.73	44.77	2
	BVRB_6g154910	Cy	231	26 331.41	5.00	38.20	2
	BVRB_6g154940	Cy	230	25 875.88	5.42	38.65	2
	BVRB_6g154920	Cy	230	25 917.99	5.25	48.93	2
	BVRB_6g154930	Cy	229	25 994.07	5.50	47.71	2
	BVRB_7g166450	Cy	223	26 048.23	6.77	49.07	2
	BVRB_7g166460	Cy	223	26 000.00	5.25	48.94	2
	BVRB_009580	Cy	224	25 974.29	5.39	27.46	2
	BVRB_010890	Cy	224	26 149.46	5.83	33.16	2
	BVRB_4g077090	Cy	217	25 447.66	5.78	47.52	1
	BVRB_7g173050	Cy	232	27 528.27	7.71	48.14	2
	BVRB_7g178130	Cy	224	25 855.05	7.55	46.87	2
	BVRB_3g069410	Cy	221	25 197.21	5.48	39.07	2
	BVRB_5g114900	Cy	230	26 316.76	5.56	43.18	2
	BVRB_5g114850	Cy	231	26 518.59	5.23	42.09	2
	BVRB_5g114830	Cy	265	30 787.11	7.60	47.22	2
	BVRB_5g114860	Cy	230	26 808.28	5.16	47，65	2
	BVRB_5g114870	Cy	224	25 783.57	4.72	44.75	2
	BVRB_5g114880	Cy	177	20 512.63	5.67	40.90	2
	BVRB_5g114890	Cy	224	25 644.48	5.19	30.24	2
	BVRB_5g114910	Cy	230	26 328.71	5.42	47.37	2
	BVRB_5g114920	Cy	227	26 049.35	6.20	26.63	2
	BVRB_005270	Cy	229	26 945.36	6.67	40.54	2
	BVRB_005280	Cy	229	26 693.17	6.71	55.67	2
	BVRB_7g165490	Cy	223	25 875.84	6.77	45.86	2
Lambda	BVRB_2g036650	Nu	136	15 295.31	4.85	45.32	4
	BVRB_1g016480	Cy	235	26 626.36	5.02	37.65	8
	BVRB_7g175450	Cy	240	28 054.85	5.22	47.73	8
DHAR	BVRB_2g039390	Cy	214	24 114.66	5.64	42.56	6
DHAR	BVRB_2g046040	Cy	212	23514.24	6.09	28.80	6

Table 2

Fig.1

Fig. 2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

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基因 Gene	上游引物 Forward primer（5′→3′）	下游引物 Reverse primer（5′→3′）
BVRB_6g151700	TAGCCGCCTCTGCTTACCTA	TGCTCCAGAAATCAAGCAACAC
BVRB_9g206010	TTGAGTTAGTACCCGTAGACTTG	GGTATTTGACCAAATGGGTTAAGGG
BVRB_6g154920	TCTGCTCCGGCCTCTATTCT	TGCAAACTTGGGAACCACTTT
BVRB_5g114870	GTCTCCCAATTGCGGAATCA	GCCCAAAGTTTCGGATATCTGTT
Actin	GCTTTGAACGACCACTTCGC	ACGCCGAGAGCAACTTGAAC

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