Relationship Between Parental Genetic Distance and Heterosis of Growth Traitsin Pinus elliottii × P.caribaea var. Hondurensis Based on SNP marker

doi:10.7525/j.issn.1673-5102.2021.05.012

Abstract

Abstract:

Using SNP loci to estimate the genetic distance between the parents of Pinus elliottii × P. caribaea， and effectively predicting the heterosis of tree height， diameter at breast height（DBH） and timber volume in their hybrids， which provides a reference for molecular marker-assisted mating design and breeding. Using SLAF-seq technology to sequence and analyze the DNAs of 131 germplasm resources from the parents of P. elliottii × P. caribaea， and valid SNP markers were obtained. Based on these SNP loci， the genetic distances between 13 parents of P. elliottii × P. caribaea were estimated by using MEGA 5.0 software， and cluster analysis were performed. The heterosis of the growth traits and the correlation between heterosis and SNP genetic distance of the 13 cross combinations were analyzed and calculated by using SPSS software. A total of 53 952 polymorphic SLAF tags and 96 736 valid SNP markers were obtained by SLAP-seq. The genetic distance between the parents of P. elliottii × P. caribaea was arranged from 0.425 1 - 0.490 6， and the correlation coefficients between SNP genetic distance of the parents and heterosis of their hybrids in tree height， DBH and timber volume were 0.680， 0.648 and 0.624， respectively. All three correlation coefficients reached a significantly positive correlation level. Utilizing SLAF-seq technology can obtain massive SNP loci. Based on these SNPs， the genetic distance between parents of P. elliottii × P. caribaea can be estimated， and the heterosis of tree height， DBH and timber volume in the hybrids can be effectively predicted.

Key words: Pinus elliottii × P.caribaea, SNP marker, genetic distance, heterosis

CLC Number:

S791.246

Yi-Liang LI, Fen-Cheng ZHAO, Yang LIU, Sui-Ying ZHONG, Chang-Ming LIN, Zhi-Qiang TAN, Fu-Ming LI, Fang-Yan LIAO, Hui-Shan WU, Wen-Bin GUO, Zhe WANG. Relationship Between Parental Genetic Distance and Heterosis of Growth Traitsin Pinus elliottii × P.caribaea var. Hondurensis Based on SNP marker[J]. Bulletin of Botanical Research, 2021, 41(5): 738-743.

Figures/Tables 5

Table 1

Fig.1

Table 2

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亲本 Parent	A06	B20	B118	SS05-2	HL09	HL17	HQ09	ZH03
A06	—
B20	0.098 0	—
B118	0.085 9	0.084 6	—
SS05-2	0.135 9	0.133 9	0.125 2	—
HL09	0.483 1	0.479 7	0.437 8	0.461 7	—
HL17	0.490 6	0.479 3	0.449 6	0.466 2	0.242 0	—
HQ09	0.459 9	0.456 4	0.425 1	0.442 1	0.251 4	0.255 4	—
ZH03	0.479 5	0.472 9	0.433 8	0.464 9	0.237 0	0.233 6	0.244 6	—

杂交组合 Cross	树高 Tree height （m）	胸径 Diameter at breast height（cm）	材积 Volume of timbet（m³）
A06×HL17	9.91	16.73	0.1118
A06×HQ09	9.15	15.06	0.0856
A06×ZH3	9.29	15.27	0.091
B118×HL09	9.30	15.14	0.0897
B118×HL17	9.25	15.15	0.0886
B118×HQ09	8.99	15.17	0.0866
B118×ZH3	9.10	15.40	0.0928
B20×HL17	9.20	14.67	0.0809
B20×HQ09	9.48	14.60	0.0872
B20×ZH3	9.71	16.27	0.1013
SS05-2×HL17	8.96	14.79	0.0831
SS05-2×HQ09	8.88	14.36	0.0816
SS05-2×ZH3	9.24	14.91	0.0862

亲本组合 Combination	树高 Tree height （m）	胸径 Diameter at breast height（cm）	材积 Volume （m³）
A06×HL17	30.45	73.58	261.74
A06×HQ09	20.39	56.26	176.90
A06×ZH3	22.17	58.45	194.41
B118×HL09	22.33	57.08	190.30
B118×HL17	21.71	57.17	186.54
B118×HQ09	18.28	57.45	180.33
B118×ZH3	19.78	59.79	200.29
B20×HL17	21.00	52.18	161.85
B20×HQ09	24.78	51.49	182.24
B20×ZH3	27.73	68.81	227.67
SS05-2×HL17	17.88	53.44	168.84
SS05-2×HQ09	16.87	49.03	163.92
SS05-2×ZH3	21.64	54.67	178.84
均值Average value	21.92	57.65	190.30

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