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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (5): 789-794.doi: 10.7525/j.issn.1673-5102.2020.05.019

• Scientific Notes • Previous Articles     Next Articles

Optimization of the ActiveCompoundsExtraction Process from Fig Leaves by Anionic and Cationic Surfactants and Preliminary Functional Evaluation

MENG Yao1,2,3, YU Lian1,2,3, QIN Xiang-Yu1,2,3, LIU Chang1,2,3, WANG Ze-Lin1,2,3, ZHAO Chun-Jian1,2,3, FU Yu-Jie1,2,3   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. Engineering Research Center of Forestry Biological Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040;
    3. Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040
  • Received:2020-04-07 Online:2020-09-05 Published:2020-07-10
  • Supported by:
    National Key R&D Program of China(2016YFD0600805);Heilongjiang Province Applied Technology Research and Development Project(GX18B003);Innovative and intelligent Projects 111(B20088)

Abstract: The experimental conditions for formulated surfactant combined with microwave-assisted extraction of effective bioactive substances in fig leaves were designed and the process parameters were optimized. The final results were as follows:1%(w/v) formulated surfactant(sodium dodecyl sulfate(SDS):dodecyl trimethyl ammonium bromide(DTAB)), extraction temperature of 40℃, extraction time of 10 min, liquid-solid ratio of 30:1 mL·g-1. Under the above optimized conditions, the average extraction yields of psoralen and bergapten were 15.37 and 3.59 mg·g-1, respectively. The antioxidant activity of crude fig extract obtained by surfactant-assisted extraction was significantly enhanced as compared with that by conventional aqueous extraction. Meanwhile, the degradation rate of the main substance in fig leaveswas also significantly inhibited. This study provides the necessary scientific basis for the rational and effective development and utilization of fig leaf resources. Also, it is of great theoretical significance for the green and efficient extraction of natural and effective target products and functional evaluation in plants.

Key words: fig leaf, surfactant, microwave-assisted extraction, single factor experiment

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