Expression Analysis of the FaesAP2B in Mutant Buckwheat with Long Pistil and Long Stamen

doi:10.7525/j.issn.1673-5102.2020.02.014

Abstract

Abstract: In order to uncover the molecular mechanism of flower and grain development of buckwheat(Fagopyrum esculentum Moench.) with long pistil and long stamen mutant ‘lpls’ of a 1 788 bp long AP2 homologous cDNA sequence was cloned from F.esculentum Moench. and designated as FaesAP2B(GenBank accession number is MK290847.1). By sequence analysis, FaesAP2B gene contains a full Open Reading Frame with 1 380 bp long, and encodes an AP2/ERF family transcription factor consisting of 459 amino acid residues. The FaesAP2B transcription factor contains two highly conserved AP2 domains, with a nuclear localization signal region consisting of ten amino acid residues lying before the first AP2 domain. qPCR was performed to detect FaesAP2B gene expression in root, stem, leaf, perianth, stamen, pistil and juvenile fruit in four-day buckwheat mutant lpls, respectively. The FaesAP2B is expressed in both vegetative tissues and reproductive structures. However, the expression level of FaesAP2B in reproductive organs such as floral organs and fruits is significantly higher than that in vegetative tissues. In addition, the expression level of FaesAP2B in stamens is the highest among seven different organs(LSD, P<0.01). And the expression level of FaesAP2B in the perianth, pistil and four-day fruit was significantly higher than that in the vegetative organs such as roots, stems and leaves(LSD, P<0.01), but there was no significant difference in the expression of the gene between roots, stems and leaves. Our data suggested that FaesAP2B may be mainly involved in regulating flower and fruit development in the buckwheat mutant ‘lpls’.

Key words: Fagopyrum esculentum Moench., flower development, FaesAP2B, AP2

CLC Number:

S517

ZHANG Jiao, WANG Xuan, ZHANG Liang-Bo, LIU Zhi-Xiong. Expression Analysis of the FaesAP2B in Mutant Buckwheat with Long Pistil and Long Stamen[J]. Bulletin of Botanical Research, 2020, 40(2): 266-273.

References 32

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