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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (6): 846-852.doi: 10.7525/j.issn.1673-5102.2019.06.006

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Cytogenetic Identification on Interspecific Hybrids in Genus Elymus L. of Qinghai Plateau

LU Xing-Wang1,2, LIU Bo1,2, LIU Rui-Juan1,3, DOU Quan-Wen1,3   

  1. 1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008;
    2. University of Chinese Academy of Sciences, Beijing 100049;
    3. Key Laboratory of Crop Molecular Breeding of Qinghai Province, Xining 810008
  • Received:2019-02-26 Online:2019-11-05 Published:2019-11-16
  • Supported by:
    The Major Science and Technology Project of Qinghai(2018-NK-A2)

Abstract: Cytogenetic identification was conducted on interspecific hybrids in genus Elymus L. spontaneously occurred in Qinghai plateau by using a technique of Fluorescence in situ hybridization(FISH) and Genomic hybridization(GISH). Combined with information of the morphology and ecology distribution of the sympatric species, the hybrids were identified as six types:Ⅰ is a hybrid between Elymus nutans Griseb. and a Roegneria C.Koch species with a chromosome number of 35 and genome constitution of StStYYH; Ⅱ is a hybrid between E.nutans and E.dahuricus Turcz. ex Griseb. with a chromosome number of 42 and genome constitution of StStYYHH. Ⅲ is a hybrid between E.sibiricus L. and E.dahuricus with a chromosome number of 35 and genome constitution of StStHHY; Ⅳ is a hybrid between E.nutans and Kengilia hirsuta Keng with a chromosome number of 42 and genome constitution of StStYYHP; Ⅴ is a hybrid between E.nutans and K.grandiglumis Keng with a chromosome number of 42 and genome constitution of StStYYHP; Ⅵ is a hybrid between K.hirsuta and Leymus secalinus(Georgi) Tzvel. with a chromosome number of 35 and genome constitution of StYPNsXm. The results provide the basic evidence for further studying the introgression hybridizations in Elymus species. The identified hybrids are also valuable germplasms for grass forage improvement in this region.

Key words: Elymus L., spontaneous hybrids, cytogenetic identification, Qinghai Plateau

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