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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (6): 820-827.doi: 10.7525/j.issn.1673-5102.2018.06.004

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Analysis on Relationship and Taxonomic Status of Some Species in Subg. Cerasus Koehne with Chloroplast DNA atpB-rbcL Fragment

ZHU Hong, YI Xian-Gui, ZHU Shu-Xia, WANG Hua-Chen, DUAN Yi-Fan, WANG Xian-Rong   

  1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Key Laboratory of State Forestry Administration on Subtropical Forest Biodiversity Conservation, Nanjing 210037
  • Received:2018-09-13 Online:2018-11-15 Published:2018-11-01
  • Supported by:
    The Three New Forestry Project of Jiangsu(LYSX[2015]17);The Postgraduate Innovation Project of Jiangsu(KYCX17_0815);The Doctorate Fellowship Foundation of Nanjing Forestry University

Abstract: Subg. Cerasus Koehne of Rosaceae families (Flowering Cherries) has important ornamental value. An in-depth research on the interspecies relationships and their taxonomic status contributes to the development of those cherry species and germplasm resource innovation. in China. The tree species of Subg. Cerasus (including variety) collected from Yunnan, Jiangxi and Fujian were amplified and sequenced, using the atpB-rbcL fragment of noncoding region in chloroplast DNA, and integrated with homologous sequences from four other species committed to database of GenBank. Haplotype of Median-Joining network and the phylogenetic tree were both constructed based on a total of 30 sequence matrix including 7 species, and their relationships and classification status were discussed. (1) There were total 15 polymorphism sites in the 774 valid sites of atpB-rbcL sequence matrix, accounting for 1.94%, and the (A+T) content 70.50%, reflecting an obvious base bias; (2) Total 9 haplotypes were detected by DNASP 5 software, with average Haplotype diversity (Hd=0.880 5±0.026) and average nueleotide diversity (π=0.007 11±0.000 54); Except for Prunus dielsiana (Hap5~Hap7), the remaining species had their own unique haplotype, which indicate abundant genetic diversity; (3) After integrating with the results of MJ network as well as reconstruction of the phylogenetic tree, we speculated that P.pananensis was during the original stage of evolution,moreover, it is closely related to P.schneideriana when compare with P.dielsiana; P.dielsiana var. longistyla was a sister branch to P.dielsiana, also together constituted an independent evolutionary unit. With the above results, we believe that the Chloroplast DNA atpB-rbcL fragment is relatively suitable at intergeneric or interspecific levels, which could be selected as DNA bar code in priority. Molecular evidence combined with morphological features support the viewpoint that regarding P.dielsiana var. longistyla as a varity of P.dielsiana, while the taxonomic status of P.pananensis calls for more evidence.

Key words: Subg. Cerasus Koehne, cpDNA atpB-rbcL, haplotype, phylogeny, taxonomic status

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