Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg.

doi:10.7525/j.issn.1673-5102.2017.06.020

Abstract

Abstract: R2R3-type MYB transcription factor participates in versatile biological process including stress response process. To identify the structure and function of the MYB gene in rubber tree, the full length cDNA of HbMYB62 was cloned in leaves of rubber tree. It was 1 013 bp in length, containing a 945 bp ORF which encodes 314 amino acid residues. The deduced amino acid sequence had the specific domains of plant HTH_MYB DNA binding domain. It showed the highest identity with AtMYB62 in all AtMYB family. This gene is mainly expressed in flower, whilst less expressed in bark, latex and leaf. The gene expression of HbMYB62 were differentially upregulated by H₂O₂, ABA, and SA. The HbMYB62 gene may play an important role in stress resistance and plant hormones signal transduction pathway in rubber tree. This study provides a fundamental knowledge for further study the structure and functions of HbMYB62.

Key words: Hevea brasiliensis, HbMYB62, wounding, hormone, gene expression

CLC Number:

S794.1

LU Yan-Xi, ZHANG Dong, WANG Li-Feng, WANG Ji-Kun. Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg.[J]. Bulletin of Botanical Research, 2017, 37(6): 953-960.

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