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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (5): 751-760.doi: 10.7525/j.issn.1673-5102.2017.05.016

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Cloning and Expression Analysis of RbohB Gene in Brassica campestris L.

ZHANG Teng-Guo, LUO Dan-Yu, GUO Yan-Feng, ZHENG Sheng, WANG Juan   

  1. School of Life Sciences, Northwest Normal University, Lanzhou 730070
  • Received:2016-12-19 Online:2017-09-15 Published:2017-09-15
  • Supported by:
    The National Natural Science Foundation of China(31460099)

Abstract: A novel RbohB gene was isolated from Brassica campestris L. Longyou 6 by RACE. The full-length cDNA of RbohB is 2 694 bp, with open reading frame (ORF) 2 541 bp, encoding a 846 amino acid protein. By RT-PCR analysis, transcript level of RbohB was increased in response to cold, high salt and H2O2 stress. However, MAPKK inhibitor U0126 pretreatment 12 h before cold, high salt and H2O2 stress, compared with the single stress, the expression level of RbohB was significantly decreasing. The results suggested that RbohB played an important role during cold, high salt and H2O2 stress in B.campestris L. and U0126 has inhibitory effect to RbohB gene transcription. The activity level of NADPH oxidase was increased in response to H2O2 stress. However, U0126 pretreatment 12 h before H2O2 stress, compared with the single H2O2 stress, NADPH oxidase activity was significantly decreasing. The result showed that MAPKK inhibitor U0126 has inhibitory effect to NADPH oxidase activity.

Key words: Brassica campestris L., RbohB gene, molecular cloning, expression analysis

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