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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (6): 870-877.doi: 10.7525/j.issn.1673-5102.2016.06.010

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Exogenous ABA Affected on Antioxidant Enzymes and Osmotic Adjustment in Choraspora bungeana Under Low Temperature Stress

HE Wen-Ping, WANG Xin-Xia, ZHANG Xu-Qiang, YANG Peng-Jun, QIU Yi-Zhi, YANG Ning   

  1. College of Life Sciences, Northwest Normal University, Lanzhou 730070
  • Received:2016-09-06 Online:2016-11-15 Published:2016-11-16
  • Supported by:
    Supported by National Natural Science Foundation of China(31360061,31660116)

Abstract: Using plantlets of Choraspora bungeana as material, researched the effect of exogenous ABA on antioxidant enzyme and osmotic regulation substances under chilling(4℃), freezing(-4℃) and critical temperature(0℃) were studied. The results showed the activities of SOD and CAT in negative control group were higher than blank control group under 4℃ and -4℃. In negative control group the POD activity was higher than blank control group under 4℃. The APX activity increased at the late period of 4℃ and -4℃ treatment in negative control group. Meanwhile the relative conductivity increased but proline and soluble sugar decreased in negative control group. At 0℃, in negative control group the POD activity decreased and the APX activity changed with fluctuation. The content of proline and soluble sugar was higher than blank control group at 72 h and 48h respectively and soluble protein was lower than blank control group under 0℃. Exogenous ABA treatment, increased the antioxidant enzymes activities with different extent under low temperature stress. For the osmotic regulation substances, exogenous ABA treatment decreased the relative conductivity and increased the content of proline, soluble sugar and soluble protein.

Key words: low temperature stress, Choraspora bungeana, abscisic acid, antioxidant enzymes, osmotic regulation substances

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