Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong)

doi:10.7525/j.issn.1673-5102.2016.05.017

Abstract

Abstract: COR genes play important roles in plant cold acclimation process. In order to study the Cor gene function of Camellia japonica to severe cold, adopting homologous cloning method, a 617 bp full-length cDNA sequence was cloned from leaves of C.japonica(Nai Dong) under the natural low temperature condition, named CjCor1. The total length sequence is complete, and contains an opening reading frame of 600 bp. Amino acids sequence alignment shows that CjCor1 gene encodes a protein of 199 amino acids, containing specific conservative domain structure of WCOR413 in cold acclimatization protein superfamily, and highly conservative property in species belonged to same genus. By real-time PCR analysis, comparing with southern Camellia species and common varieties, CjCor1 genes had higher expression in C.japonica(Nai Dong) under the condition of low temperature(below 0℃), and also had higher expression in higher cold resistance varieties.

Key words: Camellia japonica(Nai Dong), CjCor1 gene, sequence analysis, gene expression

CLC Number:

S685.14

YANG Kai, SUN Ying-Kun, WANG Kui-Ling, LIU Qing-Hua, GAO Han-Dong. Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong)[J]. Bulletin of Botanical Research, 2016, 36(5): 753-759.

References

1. 张乐初,游慕贤,陈德松.中国茶花文化[M].上海:上海文化出版社,2003:85-122.Zhang L C,You M X,Chen D S.Chinese camellia culture[M].Shanghai:Shanghai Culture Publishing House,2003:85-122.
2. 王奎玲.耐冬山茶种质资源研究[D].北京:北京林业大学,2006.Wang K L.Studies on the germplasm resources of Naidong Shancha(Camellia Japonica L.)[D].Beijing:Beijing Forestry University,2006.
3. 黄永会,朱莉莎,王玉臣,等.结球白菜COR基因克隆及序列分析[J].贵州农业科学,2014,42(11):10-13.Huang Y H,Zhu L S,Wang Y C,et al.Gene clone and sequence analysis of Brassica campestris ssp.Pekinensis[J].Guizhou Agricultural Sciences,2014,42(11):10-13.
4. Huang B,Jin L G,Liu H Y.Molecular cloning and functional characterization of a DREB1/CBF-like gene(GhDREB1L) from cotton[J].Science in China Series C:Life Sciences,2007,50(1):7-14.
5. 贺泂杰.高寒冰缘植物高山离子芥CbCBF4基因功能的研究[D].兰州:兰州大学,2012.He J J.The functional research on CbCBF4[D].Lanzhou:Lanzhou University,2012.
6. 洪剑明,邱泽生.植物抗性生理(一)[J].生物学通报,1997(5):13-15.Hong J M,Qiu Z S.Plant resistant physiology(No.1)[J].Bulletin of Biology,1997(5):13-15.
7. 王国莉,郭振飞.植物耐冷性分子机理的研究进展[J].植物学通报,2003,20(6):671-679.Wang G L,Guo Z F.The progress of researches on molecular mechanism of chilling tolerance in plants[J].Chinese Bulletin of Botany,2003,20(6):671-679.
8. 孙迎坤,李纪元,殷恒福,等.重瓣山茶花‘金盘荔枝’C功能基因CjAGL6的全长克隆与表达分析[J].植物研究,2013,33(3):330-338.Sun Y K,Li J Y,Yin H F,et al.Cloning and expression analysis of C function CjAGL6 gene cDNA from Camellia japonica ‘Jinpan Lizhi’[J].Bulletin of Botanical Research,2013,33(3):330-338.
9. 王仁卿,张淑萍,石竹,等.青岛耐冬山茶的多样性特征及其保护[J].山东林业科技,1998,(3):1-4.Wang R Q,Zhang S P,Shi Z,et al.Diversity characteristics and protection of Naidong Shancha in Qingdao[J].Journal of Shandong forestry science and technology,1998(3):1-4.
10. Mikkelsen M D,Thomashow M F.A role for circadian evening elements in cold-regulated gene expression in Arabidopsis[J].The Plant Journal,2009,60(2):328-339.
11. Kilian J,Whitehead D,Horak J,et al.The AtGenExpress global stress expression data set:protocols,evaluation and model data analysis of UV-B light,drought and cold stress responses[J].The Plant Journal,2007,50(2):347-363.
12. Xu W R,Li R M,Zhang N B,et al.Transcriptome profiling of Vitis amurensis,an extremely cold-tolerant Chinese wild Vitis species,reveals candidate genes and events that potentially connected to cold stress[J].Plant Molecular Biology,2014,86(4-5):527-541.
13. 范高韬,孙小明,任小蝶,等.葡萄COR27基因的克隆与抗寒功能研究[J].植物科学学报,2015,33(3):346-354.Fan G T,Sun X M,Ren X D,et al.Cloning and functional analysis of COR27 gene from Vitis vinifera[J].Plant Science Journal,2015,33(3):346-354.
14. 谢素霞,张媛,郭凯琳,等.一个新的茶树黄酮醇合酶基因的克隆和表达分析[J].植物生理学通讯,2009,45(11):1093-1097.Xie S X,Zhang Y,Guo K L,et al.Cloning and expression analysis of a flavonol synthase gene from Camellia sinensis[J].Plant Physiology Communications,2009,45(11):1093-1097.
15. Li X W,Feng Z G,Yang H M,et al.A novel cold-regulated gene from Camellia sinensis,CsCOR1,enhances salt- and dehydration-tolerance in tobacco[J].Biochemical and Biophysical Research Communications,2010,394(2):354-359.
16. Chen L,Zhong H,Ren F,et al.A novel cold-regulated gene,COR25,of Brassica napus is involved in plant response and tolerance to cold stress[J].Plant Cell Reports,2011,30(4):463-471.
17. Seki M,Narusaka M,Abe H,et al.Monitoring the expression pattern of 1300Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray[J].The Plant Cell,2001,13(1):61-72.
18. Breton G,Danyluk J,Charton J B F,et al.Expression profiling and bioinformatic analyses of a novel stress-regulated multispanning transmembrane protein family from cereals and Arabidopsis[J].Plant Physiology,2003,132(1):64-74.
19. 秦华,眭顺照,李名扬,等.蜡梅(Chimonanthus praecox(L.)Link)COR413蛋白基因(Cpcor413pm1)的分子特性与表达分析[J].中国生物化学与分子生物学报,2006,22(7):547-552.Qin H,Sui S Z,Li M Y,et al.Molecular characterization and expression analysis of a novel COR413 gene from Wintersweet (Chimonanthus praecox(L.) Link)[J].Chinese Journal of Biochemistry and Molecular Biology,2006,22(7):547-552.

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