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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (3): 434-443.doi: 10.7525/j.issn.1673-5102.2016.03.018

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Establishment and Optimization of plant regeneration of Hybrid larch(L.gmelinii 10×L.kaempferi 13)

ZHANG Li, ZHANG Lei, HOU Dan, ZHANG Su-Fang, ZHANG Han-Guo   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2015-10-27 Online:2016-05-15 Published:2016-05-20

Abstract: With the mature zygotic embryos of hybrid larch(L.gmelinii 10×L.kaempferi 13), by combining different culture medium and hormone, we established and optimized hybrid larch mature zygotic embryos induction and plant regeneration. BM medium was suitable for adventitious bud induction of hybrid larch with the induction rate of 60.7%. Under the condition of combination BM and1.5 mg·L-1 TDZ, induction rate was the highest, 67.73%. BM medium could promote the proliferation of adventitious buds with the value-added coefficient of 4.48, when it was cultured on BM+1.0 mg·L-1 TDZ with the coefficient of 5.23. The active carbon could promote the elongation at the level of 2 g·L-1. When the adventitious buds were cultured under BM+0.05 mg·L-1 6-BA+0.2 mg·L-1 NAA, the pumping stems rate was the highest, up to 73.96%, and the highest rooting rate was 29.85% under 1/2BM+0.5mg·L-1IBA+1.0mg·L-1 NAA.

Key words: hybrid larch, the mature zygotic embryos, adventitious buds, tissue culture

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