Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata

doi:10.7525/j.issn.1673-5102.2016.03.015

Abstract

Abstract: We studied the effects on the key signal molecules and the main secondary metabolites's content in tissue culture of Rhodiola crenulata inoculated with endophic fungi ZPRa-R-1. We selected Greiss method, RP-HPLC and Titanium sulfate method to detect the contents of NO, SA and H₂O₂, respectively, and detected the key enzymes, PAL and CA4H by spectrophotometry. p-Tyrosol and salidroside in Rhodiola seedling co-cultured with fungi were evaluated by RP-HPLC. The content of NO increased firstly, followed by SA and H₂O₂ after that Rhodiola seedling inoculated with endophic fungi. The maximum contents of NO, SA and H₂O₂ were detected at 8, 10 and 12 d, and they were 0.193 μmol·g^-1, 0.062 μg·g^-1 and 0.092 μmol·g^-1, respectively. The maximum contents of PAL and CA4H were 166.400 U·g^-1, 0.625 U·g^-1·h^-1, and were 8 and 1.5 times as much as that of control, repectively. The contents of p-tyrosol and salidroside were accumulated, and the time of each of them reaching the maximum content was on 8 and 10 d, and the contents were 8.656 and 0.498 mg·g^-1, and were 7 and 2.5 times as much as that of control, respectively. Therefore, ZPRa-R-1 has the capacity of regulating signals and promoting the activities of PAL and CA4H, and finally promoted accumulation of p-tyrosol and salidroside in co-culturing seedling of fungi and Rhodiola.

Key words: Rhodiola crenulata, endophytic fungi, signal molecule, p-tyrosol, salidroside

CLC Number:

S567

WANG Meng-Liang, JIAO Jin, XING Jie, TIAN Jun-Sheng, CUI Jin-Long, WANG Jun-Hong. Effects of Endophytic Fungi ZPRa-R-1 on the Key Singnal Molecules and the Main Secondary Metabolites in Rhodiola crenulata[J]. Bulletin of Botanical Research, 2016, 36(3): 416-420.

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