Molecular and Cytological analysis of Meiosis in Autotetraploid Arabidopsis thaliana

doi:10.7525/j.issn.1673-5102.2016.03.006

Abstract

Abstract: The diploid Arabidopsis thaliana(Columbia-0, 2n=10) was used to obtain autotetraploid plants(2n=20) after 0.2% colchicine treatment and cytological identification. Meiotic chromosome behaviors were comparatively observed in diploid and autotetraploid A.thaliana, and the meiosis-related genes were relatively quantified by Real-time PCR. The autotetraploid A.thaliana produced relatively larger stoma and fruits, but the stomatal density and the seed-set were significantly reduced. Some chromosomal configurations were also observed during meiosis involving the trivalents, univalents, bivalents and quadrivalents in autotetraploid plants. The expression of several meiosis-related genes including ASY1, DMC1, MRE11 and SPO11-1 were up-regulated in autotetraploids. Therefore, as autopolyploidization proceeds, plants may adapt themselves into environment by accompanying with phenotype changes owing to the altered chromosomal behaviors and gene expressions.

Key words: Arabidopsis thaliana, diploid, autotetraploid, meiosis, chromosome

CLC Number:

Q943

LI Yun-Ling, TIAN Bao-Ming, YANG Yan, CUI Ming-Zhu, HAO Zhi-Da, WEI Fang. Molecular and Cytological analysis of Meiosis in Autotetraploid Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2016, 36(3): 354-359.

References

1. 张振超,张蜀宁,张伟,等. 四倍体不结球白菜的诱导及染色体倍性鉴定[J].西北植物学报,2007,27(1):28-32.
2. 何婷,刘成洪,杜志钊,等. 气孔保卫细胞大小与油菜单倍体及二倍体倍性的相关性研究[J].上海农业学报,2012,28(4):42-45.
3. 刘惠吉,张蜀宁,王华. 青梗、优质、抗热同源四倍体白菜杂交新品种暑优1号选育[J].南京农业大学学报,2002,25(3):22-26.
4. 崔群香,王倩,唐红艳,等. 普通白菜同源四倍体花粉母细胞减数分裂及其雄配子体发育研究[J].扬州大学学报,2012,33(4):56-60.
5. 张新全,杜逸,郑德成. 鸭茅二倍体和四倍体PMC-减数分裂、花粉育性及结实性的研究[J].中国草地,1996,6:38-40.
6. 刘洋,何心尧,马红波,等. 用CTAB-PVP法提取棉花各组织总RNA的研究[J].中国农业大学学报,2006,11(1):53-56.
7. Pecinka A,Fang W,Rehmsmeier M,et al. Polyploidization increases meiotic recombination frequency in Arabidopsis[J].BMC Biology,2012,9:24.
8. 万正林,龙明华,邓俭英,等. 黑皮冬瓜同源四倍体与起源二倍体农艺性状比较[J].南方农业学报,2014(9):1621-1625.
9. 于文艳,刘世琦,梁庆玲,等. 大蒜二倍体与四倍体生长和生理指标的比较研究[J].山东农业科学,2008(2):42-44.
10. 李红,杨風,向增旭. 甜叶菊同源四倍体离体诱导及鉴定[J].西北植物学报,2012,32(8):1692-1697.
11. 刘静,赵庆芳,丁兰. 兰州百合多倍体诱导及鉴定[J].北方园艺,2011(18):138-141.
12. Mohammadi P P,Moieni A,Ebrahimi A,et a1. Doubled haploid plants following colchicine treatment of microspore derived embryos of oilseed rape(Brassica napus L. )[J].Plant Cell Tissue Organ Culture,2012,108:251-256.
13. Miller M,Zhang C,Chen Z J. Ploidy and hybridity effects on growth vigor and gene expression in Arabidopsis thaliana hybrids and their parents[J].G3:Genes Genomes Genet,2012,4(2):505-513.
14. 吴改娥,刘平,刘孟军,等. 二倍体和同源四倍体"辣椒枣"减数分裂的比较[J].园艺学报,2011,38(5):867-870.
15. 王惠利,赵晓明. 二倍体与四倍体金银花减数分裂观察[J].江苏农业科学,2014,42(9):211-214.
16. Caryl A P,Armstrong S J,Jones G H,et al. A homologue of the yeast HOP1 gene is inactivated in the Arabidopsis meiotic mutant ASY1[J].Chromosoma,2002,109(1-2):62-71.
17. Armstrong S J,Caryl A P,Jones G H,et al. ASY1,a protein required for meiotic chromosome synapsis,localizes to axis-associated chromatin in Arabidopsis and Brassica[J].Journal of Cell Science,2002,115(18):3645-3655.
18. Eugenio S M,Santos J L,Gareth H,et al. ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis[J].Genes & Development,2007,21:2220-2233.
19. Li W X,Chen B C,Markmann M U,et al. The Arabidopsis AtRAD51gene is dispensable for vegetative development but required for meiosis[J].Proc Nat Acad Sci USA,2004,101(29):10596-10601.
20. Siaud N,Dray E,Gy I,et al. Brca2 is involved in meiosis in Arabidopsis thaliana as suggested by its interaction with DMC1[J].EMBO Journal,2004,23(6):1392-1401.
21. Higgins J D,Sanchez M E,Armstrong S J,et al. The Arabidopsis synaptonemal complexprotein ZYP1 is required for chromosome synapsis and normal fidelity of crossing over[J].Genes & Development,2005,19(20):2488-2500.
22. James D Higgins,Eugenio S M,et al. MRE11 deficiency in Arabidopsis is associated with chromosomal instability in somatic cells and SPO11-dependent genome fragmentation during meiosis[J].Plant Cell,2004,16(8):1968-1978.
23. Wang M,Wang K J,Tang D,et al. The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice[J].Plant Cell,2010,22(2):417-430.
24. Bleuyard J Y,Gallego M E,White C I. Meiotic defects in the Arabidopsis rad50 mutant point to conservation of the MRX complex function in early stages of meiotic recombination[J].Chromosoma,2004,113(4):197-203.
25. Franklin. The Arabidopsis synaptonemal complex protein ZYP1 is required for chromosome synapsis and normal fidelity of crossing over[J].Genes & Development,2005,19:2488-2500.

[1]	Xiayu HU, Yuping LIU, Xu SU, Ping YANG, Yanan WANG. Chromosome Number and Karyotype Analysis of Different Populations in Sophora alopecuroides (Fabaceae) [J]. Bulletin of Botanical Research, 2023, 43(1): 9-19.
[2]	Ying WANG, Yujie ZHANG, Zihui JIAO, Xueli LI, Kai WANG, Xiaowei DA, Kun SUN, Hui ZHANG. Preliminary Study on Cross Fertility in ‘Kushui’ Rose [J]. Bulletin of Botanical Research, 2022, 42(3): 403-411.
[3]	Yi-Lan CHEN, Hai-Min LIAO, Zheng-Feng WANG, Xiang-Xun HUANG, Dong-Ming LIU. Chromosome Preparation and Karyotype Analysis of Cordia subcordata [J]. Bulletin of Botanical Research, 2021, 41(5): 684-689.
[4]	DING Hai-Yan. Fast Manufacture of Meiotic Chromosome Specimens of the Plant Material [J]. Bulletin of Botanical Research, 2017, 37(5): 797-800.
[5]	YANG Liu-Hui, ZHANG Jian-Jun, WANG Qi, ZHU Wei, ZHANG Teng, YU Xiao-Nan. Ploidy Identification and Karyotype Analysis of Five Itoh Hybrid Peonies [J]. Bulletin of Botanical Research, 2017, 37(4): 535-541.
[6]	WU Jie, ZHAO Yu-Ping, HE Xiang-Feng, WANG Wen-He, LENG Ping-Sheng, ZHANG Ke-Zhong. Cytological Study on Microspore Abortion in OT Hybrid Lily Cultivars ‘Cocossa’ [J]. Bulletin of Botanical Research, 2017, 37(1): 15-22.
[7]	WANG Yu-Jiao, LIU Jian-Xin, YU Xiao-Nan. Meiosis Observation and Comparison of Pollen Mother Cells of Triploid Paeonia lactiflora Cultivars [J]. Bulletin of Botanical Research, 2016, 36(3): 341-347.
[8]	SUN Peng;LI Yun*;JIANG Jin-Zhong;YUAN Cun-Quan;ZHANG Li-Ling. Progress of Variations in Floral Organs and Seeds of Autotetraploid Plants [J]. Bulletin of Botanical Research, 2011, 31(2): 249-256.
[9]	YAN Xiao-Dan;ZHANG Yan-Ming;LI Ji-Lin. Elytrigia Genome Structure of Hybrid Octoploid Trititrigia and Agropyron glaucum* [J]. Bulletin of Botanical Research, 2010, 30(2): 197-201.
[10]	HE Yang-Yan;MA Dan-Wei;LI Qun;WANG Ya-Nan;TIAN Jia-Qiao;CHENG Wei-Wei. Temperature Influences on the Allelopathy Effect of Aqueous Extracts from Trifolium repens* L. [J]. Bulletin of Botanical Research, 2010, 30(2): 243-247.
[11]	WU Jian-Bo;CHEN Cheng-Bin;BAO Xiao-Ying;SONG Wen-Qin;ZHAO Nian-Xi;GAO Yu-Bao. Chromosome Numbers and Karyotypes of Stipa baicalensis,Stipa grandis* and Stipa krylovii in Inner-mongolia Steppe [J]. Bulletin of Botanical Research, 2009, 29(5): 534-538.
[12]	CHANG Zhao-Yang;LI Bin;SHI Fu-Chen;. The Chromosomes and Karyotypes of Some Species in Caragana from China [J]. Bulletin of Botanical Research, 2009, 29(1): 18-24.
[13]	HU Feng-Rong;LIU Guang-Xin;XI Meng-Li;WU Zhu-Hua;SHI Ji-Sen. Chromosome Giemsa C-banding Analysis of Lilium leucanthum*(Baker) Baker [J]. Bulletin of Botanical Research, 2009, 29(1): 12-14.
[14]	ZHANG Xin-Yu;WANG Zan;ZHAO Mao-Lin;GAO Hong-Wen;LIU Dao-Lin. Observations on the Pollen Morphology and Meiosis of Pollen Mother Cell in Galega* [J]. Bulletin of Botanical Research, 2008, 28(5): 540-546.
[15]	CHEN Xia;XING Lu-Lu;DING Hai-Yan;XU Ying-Bo;LI Ji-Lin*. Study on Meiosis Comparison of Pollen Grain of F₁ Hybrided by Gametocidal Substitution Lines and Wheat [J]. Bulletin of Botanical Research, 2008, 28(4): 392-395.