Identification of Protein Interactions between Flowering Repressors BpFLC and BpSVP from Betula platyphylla

doi:10.7525/j.issn.1673-5102.2015.06.013

Abstract

Abstract: The experiment was conducted to study the mechanism of interaction between SVP and FLC in Betula platyphylla. The truncated genes of BpFLC1-6 and BpSVP1-6 were, respectively, cloned from yeast recombination plasmids pGBKT7-BpFLC and pGBKT7-BpSVP. pGBKT7-BpFLC1-6×pGADT7-BpSVP and pGBKT7-BpFLC×pGADT7-BpSVP1-6 were co-transferred into yeast Y2HGold. The yeast strains of Y2HGold［pGBKT7-BpFLC2-5×pGADT7-BpSVP］ could grew on selective agar plates TDO, QTO/A and QDO/X/A with blue stains. BpSVP truncated forms and BpFLC2-5 protein could act with each other to form heterodimers. Then, Y2HGold［pGBKT7-BpFLC×pGADT7-BpSVP2-5］ were brought into proximity to form protein compounds and activate transcription of four independent reporter genes of AUR1-C, HIS3, ADE2 and MEL1. The interactions between BpFLC3 and BpSVP3 were tested to confirm the acting domains. The yeast Y2HGold［pGBKT7-BpFLC3×pGADT7-BpSVP3］ exhibited blue stains on selective agar plates QDO/X/A. The K domain of BpFLC and that of BpSVP were the key structure domains and mediated the protein interactions between BpFLC and BpSVP.

Key words: Betula platyphylla, BpFLC, BpSVP, yeast two-hybrid, truncated form, protein interaction

CLC Number:

ZANG Li-Na；ZHENG Tang-Chun；DAI Li-Juan；LIU Cai-Xia；QU Guan-Zheng*. Identification of Protein Interactions between Flowering Repressors BpFLC and BpSVP from Betula platyphylla[J]. Bulletin of Botanical Research, 2015, 35(6): 866-872.

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