Abstract: The effects of different factors on callus induction and its regeneration system were studied by using Jiangxi Yanshan red bud taro (Colocasia esculenta L. Schott var. cormosus CV. Hongyayu) virus-free plantlet corms as test materials. The results showed: the best culture medium of callus induction of red bud taro virus-free plantlet corms was MS+TDZ 2 mg·L^-1+2,4-D 1 mg·L^-1; The best culture medium of callus differentiation of red bud taro virus-free plantlet corms was MS+TDZ 2 mg·L^-1+NAA 1 mg·L^-1; The best rooting medium of red bud taro virus-free plantlet adventitious buds was 1/2MS+NAA 0.1 mg·L^-1+PP₃₃₃ 0.1 mg·L^-1. The best transplanting matrix of plantlets regenerated from red bud taro virus-free plantlet corm calli was rotted sawdust after fermentation; The best PP₃₃₃ concentration of plantlets regenerated from red bud taro virus-free plantlet corm calli for transplanting was 20-50 mg·L^-1. The regeneration system from red bud taro virus-free plantlet corms was successfully established, which establishs foundation for transgenic technology and germplasm innovation of Jiangxi Yanshan red bud taro virus-free plantlets.

Key words: Jiangxi Yanshan red bud taro(Colocasia esculenta L. Schott var. cormosus CV. Hongyayu), virusfree plantlet, corm, callus, regeneration system