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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (4): 494-498.doi: 10.7525/j.issn.1673-5102.2013.04.018

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Ionic Liquids-based Microwave-assisted Extraction of Biochanin A and Genistein from Dalbergia odorifera T.Chen Leaves

MA Fei-Yue;DUAN Kai-Fang;WANG Xi-Qing;QIN Ming-Cong;LI Ji;FU Yu-Jie*   

  1. 1.State Engineering Laboratory for BioResource EcoUtilization,Northeast Forestry University,Harbin　150040；2.College of Life Science,Northeast Forestry University,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-07-20 Published:2013-07-20
  • Contact: FU Yu-Jie
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Abstract: In the present research, single-factor experiments and a three-variable, three-level Box-Behnken design were performed to study the ionic liquidsbased microwave-assisted extraction of biochanin A and genistein from D. odorifera T. Chen leaves. The optimum parameters were: 1.00 mol·L-1［C4MIM］Br, extraction temperature 56℃, liquid/solid ratio 18∶1 mL·g-1, extraction time 11 min, irradiation power 300 W. Under the optimal conditions, the average extraction yields of biochanin A and genistein were 1.598 and 0.939 mg·g-1, respectively.

Key words: Dalbergia odorifera T.Chen leaves, biochanin A, genistein, ionic liquids-based microwave-assisted extraction, Box-Behnken design

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