Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (5): 563-568.doi: 10.7525/j.issn.1673-5102.2011.05.009

Previous Articles     Next Articles

Effect of N+ Implantation on the Inducement of Polyploid Astragalus membranaceus(Fisch.) Bge. var. mongholicus(Bge.) Hsiao

ZHANG Li-Zhen;LI Qian-Zhong*;YANG Lin-Yuan;WU Cheng-Yan   

  1. School of Physical Science and Technology,Inner Mongolia University,Hohhot 010021
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20
  • Contact: LI Qian-Zhong
  • Supported by:
     

Abstract: A new method of improving induction rate of polyploid through chemical induction combined with physical mutation were proposed by using the diploid Astragalus membranaceus(Fisch.) Bge. var. mongholicus(Bge.) Hsiao seeds as the materials and N+ beam as the mutagenic agent. The results indicated that N+ implantation showed significant biological effects. The effects of N+ implantation combined with colchicine on inducing polyploidy of Astragalus were obvious, 44.4% doubling rate was obtained by being treated by the combination of N+ ion implantation dose 2.6×1016 N+/cm2 and colchicine concentration 100 mg·L-1 for 5 d. The best result of 46.2% was obtained by using the combination of N+ implantation dose 5.2×1016 N+/cm2 and colchicine concentration 100 mg·L-1 for 10 d. They were higher than the control group of maximum induction rate 13.9% of colchicine concentration 100 mg·L-1, treatment time 15 d. Cell chromosome identification results showed that the polyploid is tetraploid.

Key words: Astragalus membranaceus(Fisch.) Bge. var. mongholicus(Bge.) Hsiao, N+ implantation, colchicine, polyploidy induction

CLC Number: