Abstract: Acanthopanax senticosus (Rupr. et Maxim) Harms belongs to Araliaceae, which is a popular plant medicine with lots of bioactive substance around the world. Eleutheroside has many functions, such as resisting weariness and senescence, restraining tumor growth etc. Syringin is a main component of eleutheroside. Ultrasonic extraction method with 1.5 hour, sixty percent carbinol liquor as extractant and 55℃ are the best combination of extraction method. HPLC suitable condition was 266 nm as measuring wavelenghth, carbinol and water(28:72) used as mobile phase, 1 mL·min^-1 as velocity of flow, column temperature with 25℃. Syringin content in root and stem were compared and analyzed in similar habitats in Heilongjiang different zones. The results showed that in the seven investigation zones, syringin content of this species was highest in Wuying National Nature Reserve, which grew under Pinus koraiensis original forest, but lowest in Snow Hollow Beauty Spot of Wudalianchi and Miror Lake area of Mudan river. This result would be benefit on gathering many-prickle acanthopanax and selecting the cropping habitats.

Key words: Acanthopanax senticosus, syringin, HLPC