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Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (4): 473-476.

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Application of RAPD markers to identification of Vitis amurensis germplasm

WANG Jun1, GE Yu-Xiang1, HE Pu-Chao2   

  1. 1. Northeast Forestry University, Harbin 150040;
    2. Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100
  • Received:2004-02-20 Online:2004-12-15 Published:2016-06-14

Abstract: High quality genomic DNA was obtained by the method of modified CTAB.Seven germplasms Vitis amurensis were identified by RAPD, four primers screened from 30 primers could amplify clearly and stably 30 bands, average was 7.5 bands.There in 21 of 30 bands were examined for random amplified polymorphic DNA amplification patterns.They occupied 70%.Every primer could amplify different bands, the range of the number of bands was from 6 to 9.The result indicated that polymorphic band amplified by the 4 primerswas effective in identification 7 germplasms of Vitis amurensis.

Key words: Vitis amurensis Rupr., germplasm, identification, RAPD