Study on the factors affecting Agrobacterium-mediated transformation in Aloe (A.arborescens)

Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (1): 125-128.

Study on the factors affecting Agrobacterium-mediated transformation in Aloe (A.arborescens)

CHEN Ting-Su¹, ZHANG Jun², XIA Ning-Shao², CHEN Ru-Kai³, LI Yang-Rui⁴

1. Biotechnology Research Institute, Guangxi Academy of Agriculture Sciences, Nanning 530007;
2. Tumour Cell Engineering Lab, Xiamen University, Xiamen 361005;
3. Fujian Agriculture and Forestry University, Fuzhou 350002;
4. Guangxi Key Lab for Crop Improvement and Biotechnology, Nanning 530007

Received:2003-05-08 Online:2004-03-15 Published:2016-06-14

Abstract

Abstract: Susceptibility of the acceptors to antibiotics and transient expression of β-glucuronidase (Gus) gene were used to determine the optimized conditions for transformation of transverse thin cell layer (tTCL)of aloe (A.arborescens)mediated by Agrobacterium Tumefaciens.The results showed that both cefotaxime and carbenicillin were insusceptible while both kanamycin and hygromycin were rather sensitive to aloe.The transverse thin cell layer (tTCL)from top-part of the bud had higher percentage of shoot generation and Gus gene transient expression.Acetosyringone (AS)was necessary in aloe genetic transformation.

Key words: Aloe, Agrobacterium Tumefaciens, genetic transformation, transverse thin cell layer

CHEN Ting-Su, ZHANG Jun, XIA Ning-Shao, CHEN Ru-Kai, LI Yang-Rui. Study on the factors affecting Agrobacterium-mediated transformation in Aloe (A.arborescens)[J]. Bulletin of Botanical Research, 2004, 24(1): 125-128.

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Study on the factors affecting Agrobacterium-mediated transformation in Aloe (A.arborescens)

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