Abstract: Several surveys were carried out under Pinus yunnanensis Franch forest in Central Yunnan; the total collections are over 130. 33 species, belonging to 19 genera, 13 families were selected to supply a diversity of pure cultures. 4 types of media were selected for the pure culture study of ectomycorrhizal fungi. As a result, pure cultures of 25 species, 17 genera, and 11 families were obtained. The total isolation success rate reached 75.8%; the success rate of 4 media (No.1, No2, No.3, No.4) was 36.4%, 63.6%, 33.3% and 57.6% respectively. Modified PDA medium (No.2) and pine needles medium (No.4) are more suitable for the isolation, purification and maintenance of ectomycorrhizal fungi associated with P. yunnanensis.

Key words: Pinus yunnanensis Franch, ectomycorrhizal fungi, pure culture