Abstract: The influencing factors of RAPD were studied and the experimental parameters were optimized using Korean Pine (Pinus koraiensis) and Mongolian Oak(Quercus mongolica). The optimal experiment conditions are as follow: 20 ul system containing 10~80ng template,150~300mmol/L dNTP, 15ng arbitary primer, 1~2 units Taq DNA polymerase. With a M.J. Thermal Cycler, optimal amplification program is 40 cycles of 1 min at 94℃, 1 min at 35~37℃, 1 min at 72℃, using block control style. A high reproducibility was obtained with the optimized experiment conditions.

Key words: Random Amplified Polymorphic DNA, Influencing factors, Optimization